Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

• Published in: Agricultural biotechnology (Pawtucket, R.I.) Vol. 1; no. 3; pp. 20 - 22
• Main Authors: Peng, G, Xin, X, Che, Y, Zhang, Z, Shi, S
• Format: Journal Article
• Language: English
• Published: 01.06.2012
• Subjects: 6-BA; Hemerocallis; 大花萱草; 快速繁殖方法; 愈伤组织; 月季; 生根培养基; 组织培养; 花梗
• ISSN: 2164-4993

[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS ＋ 2.0 - 3.0 mg/L 6-BA ＋ 0.2 - 0.3 mg/L NAA, MS ＋ 1.0 - 2.0 mg/L 6-BA ＋ 0.1 - 0.2 mg/L NAA, MS and 1/2MS ＋ 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.