辐射诱变育成的黑芝麻品种赣芝9号DNA变异的SRAP分析

赣芝9号是我国首个通过辐射诱变育种技术选育而成的黑芝麻品种。利用SRAP标记对赣芝9号及其对照种武宁黑进行分子鉴定,结果显示,23对随机引物共扩增出DNA条带625条,多态性带36条,多态性比率5.76%;其中,赣芝9号新增带15条,占2.48%,缺失带21条,占对照的3.44%。辐射诱变选育的黑芝麻新品种赣芝9号与原始对照种武宁黑之间存在明显的分子变异,辐射诱变是一种有效的黑芝麻育种途径,SRAP对芝麻辐射诱变的分子鉴定是可行的。...

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Published in江西农业学报 Vol. 26; no. 10; pp. 1 - 4
Main Author 孙建 涂玉琴 乐美旺 饶月亮 颜廷献 颜小文 周红英
Format Journal Article
LanguageChinese
Published 江西省农业科学院 作物研究所,江西 南昌,330200 2014
Subjects
DNA变异
分子鉴定
赣芝9号
辐射诱变
黑芝麻
Ganzhi No.9
DNA variation
分子鉴定
Black sesame
辐射诱变
赣芝9号
Molecular identification
黑芝麻
DNA变异
Radiation mutation
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Summary:赣芝9号是我国首个通过辐射诱变育种技术选育而成的黑芝麻品种。利用SRAP标记对赣芝9号及其对照种武宁黑进行分子鉴定,结果显示,23对随机引物共扩增出DNA条带625条,多态性带36条,多态性比率5.76%;其中,赣芝9号新增带15条,占2.48%,缺失带21条,占对照的3.44%。辐射诱变选育的黑芝麻新品种赣芝9号与原始对照种武宁黑之间存在明显的分子变异,辐射诱变是一种有效的黑芝麻育种途径,SRAP对芝麻辐射诱变的分子鉴定是可行的。
Bibliography:36-1124/S
Black sesame; Ganzhi No. 9; Radiation mutation; DNA variation; Molecular identification
SUN Jian,TU Yu -qin,LE Mei -wang,RAO Yue -liang,YAN Ting- xian,YAN Xiao -wen,ZHOU Hong- ying（Crop Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China）
Ganzhi No. 9 is a first black sesame variety obtained through radiation mutation breeding in China. In this study, SRAP markers were used to conduct molecular identification of Ganzhi No. 9 and the control variety Wuninghei, and the results showed that： a total of 625 DNA bands were amplified by randomly - selected 23 pair of primers, and among them 36 bands were polymorphic with the ratio of 5.76% ; the novel bands and absent bands of Ganzhi No. 9 were 15 and 21 respectively, accounting for 2.48% and 3.44% of the total 625 bands, respectively; there was obvious molecular variation between Ganzhi No. 9 and Wun- inghei. Radiation mutation is an effective approach for black sesame breeding, and molecular identification of sesame radiati
ISSN:1001-8581