黄柏水提物联合抗菌药对产ESBLs大肠杆菌的抑菌效果

【目的】探讨黄柏水提物与抗菌药联用对产超广谱β-内酰胺酶（ESBLs）大肠杆菌的抑制效果,为黄柏的综合开发利用提供参考依据。【方法】采用二倍微量肉汤稀释法测定黄柏水提物与抗菌药的最小抑菌浓度（MIC）,并采用微量棋盘稀释法测定黄柏水提物与抗菌药联用后的分级抑菌浓度指数（FICI）。【结果】黄柏水提物对产ESBLs大肠杆菌的MIC为500 mg/m L,黄柏水提物与头孢曲松钠、阿莫西林、痢菌净、磺胺间甲氧嘧啶、头孢噻呋钠、头孢他啶、氟苯尼考、环丙沙星、诺氟沙星、左旋氧氟沙星、克林沙星、加替沙星、头孢噻肟联用后的FICI为0.50~2.01,其中,黄柏水提物与阿莫西林、头孢曲松钠、头孢噻呋钠、头孢...

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Bibliographic Details
Published in南方农业学报 Vol. 47; no. 3; pp. 500 - 505
Main Author 徐素萍 刘增援 吴永继 梁政 潘婕 孙燕杰 宋剑武 黄凯 司红彬
Format Journal Article
LanguageChinese
Published 广西大学 动物科学技术学院,南宁,530005 2016
Subjects
FICI
产ESBLs大肠杆菌
微量棋盘稀释法
抗菌药
联合用药
黄柏
联合用药
checkerboard microdilution method
drug combination
ESBLs-producing Escherichia coli
黄柏
抗菌药
产ESBLs大肠杆菌
FICI
Phellodendron amurense Rupr
antibacterial
微量棋盘稀释法
fractional inhibitory concentration index (FICI)
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Summary:【目的】探讨黄柏水提物与抗菌药联用对产超广谱β-内酰胺酶（ESBLs）大肠杆菌的抑制效果,为黄柏的综合开发利用提供参考依据。【方法】采用二倍微量肉汤稀释法测定黄柏水提物与抗菌药的最小抑菌浓度（MIC）,并采用微量棋盘稀释法测定黄柏水提物与抗菌药联用后的分级抑菌浓度指数（FICI）。【结果】黄柏水提物对产ESBLs大肠杆菌的MIC为500 mg/m L,黄柏水提物与头孢曲松钠、阿莫西林、痢菌净、磺胺间甲氧嘧啶、头孢噻呋钠、头孢他啶、氟苯尼考、环丙沙星、诺氟沙星、左旋氧氟沙星、克林沙星、加替沙星、头孢噻肟联用后的FICI为0.50~2.01,其中,黄柏水提物与阿莫西林、头孢曲松钠、头孢噻呋钠、头孢他啶、头孢噻肟、痢菌净联用存在相加作用,与氟苯尼考联用存在协同作用,与诺氟沙星、左旋氧氟沙星、克林沙星、磺胺间甲氧嘧啶间为无关作用,与环丙沙星、加替沙星联用呈拮抗作用。【结论】黄柏水提物与青霉素类、第三代头孢菌素、痢菌净、氟苯尼考联用是通过相加或协同作用提高药物对产ESBLs大肠杆菌的抑制效果,但不宜与喹诺酮类抗菌药联合用药。
Bibliography:Objective】The present experiment was conducted to investigate antibacterial effects of Phellodendron amurense Rupr water extracts combined with antimicrobials on ESBLs-producing Escherichia coli, in order to provide reference for comprehensive exploitation and utilization of P. amurense. 【Method】The minimal inhibitory concentration（MIC）of P. amurense water extracts combined with antibacterial agents were determined by double broth microdilution method.And the fractional inhibitory concentration index（FICI） of P. amurense water extracts combined with antibacterial agents to were determined by checkerboard microdilution method. 【Result】The MIC of P. amurense water extracts was 500 mg/m L.The FICI of P. amurense water extracts combined with ceftriaxone sodium, amoxicillin, mequindox, sulfamonomethoxine,ceftiofur sodium, ceftazidime, florfenicol, ciprofloxacin, norfloxacin, levofloxacin, clinafloxacin, gatifloxacin and cefotaxime ranged from 0.50 to 2.01. There was additive effect between P. amurense water extrac
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.03.500