Photobiomodulation therapy in improvement of harmful neural plasticity in sodium salicylate-induced tinnitus

• Published in: PloS one Vol. 19; no. 4; p. e0296607
• Main Authors: Montazeri, Katayoon, Farhadi, Mohammad, Majdabadi, Abbas, Akbarnejad, Zainab, Fekrazad, Reza, Shahbazi, Ali, Mahmoudian, Saeid
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.04.2024; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Biology and Life Sciences; Brain; Care and treatment; Cochlear Nucleus; Diagnosis; Engineering and Technology; Immunohistochemistry; Low-Level Light Therapy; Medicine and Health Sciences; Neuronal Plasticity - physiology; Neuroplasticity; Patient outcomes; Phototherapy; Physical Sciences; Rats; Research and Analysis Methods; Sodium Salicylate - pharmacology; Tinnitus; Tinnitus - chemically induced; Tinnitus - radiotherapy; Iran
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0296607

Tinnitus is a common annoying symptom without effective and accepted treatment. In this controlled experimental study, photobiomodulation therapy (PBMT), which uses light to modulate and repair target tissue, was used to treat sodium salicylate (SS)-induced tinnitus in a rat animal model. Here, PBMT was performed simultaneously on the peripheral and central regions involved in tinnitus. The results were evaluated using objective tests including gap pre-pulse inhibition of acoustic startle (GPIAS), auditory brainstem response (ABR) and immunohistochemistry (IHC). Harmful neural plasticity induced by tinnitus was detected by doublecortin (DCX) protein expression, a known marker of neural plasticity. PBMT parameters were 808 nm wavelength, 165 mW/cm2 power density, and 99 J/cm2 energy density. In the tinnitus group, the mean gap in noise (GIN) value of GPIAS test was significantly decreased indicated the occurrence of an additional perceived sound like tinnitus and also the mean ABR threshold and brainstem transmission time (BTT) were significantly increased. In addition, a significant increase in DCX expression in the dorsal cochlear nucleus (DCN), dentate gyrus (DG) and the parafloccular lobe (PFL) of cerebellum was observed in the tinnitus group. In PBMT group, a significant increase in the GIN value, a significant decrease in the ABR threshold and BTT, and also significant reduction of DCX expression in the DG were observed. Based on our findings, PBMT has the potential to be used in the management of SS-induced tinnitus.