Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

• Published in: Journal of Clinical Microbiology Vol. 45; no. 3; pp. 1049 - 1052
• Main Authors: Luna, Luciano Kleber de Souza, Heiser, Volker, Regamey, Nicolas, Panning, Marcus, Drexler, Jan Felix, Mulangu, Sabue, Poon, Leo, Baumgarte, Sigrid, Haijema, Bert Jan, Kaiser, Laurent, Drosten, Christian
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2007
• Subjects: Bacterial Typing Techniques; Biological and medical sciences; Coronavirus - classification; Coronavirus - genetics; Coronavirus - isolation & purification; Coronavirus 229E, Human - classification; Coronavirus 229E, Human - genetics; Coronavirus 229E, Human - isolation & purification; Coronavirus OC43, Human - classification; Coronavirus OC43, Human - genetics; Coronavirus OC43, Human - isolation & purification; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Medical sciences; Microbiology; Oligonucleotide Array Sequence Analysis - methods; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - analysis; RNA, Viral - genetics; SARS coronavirus; SARS Virus - classification; SARS Virus - genetics; SARS Virus - isolation & purification; Species Specificity; Virology; Microbiology; Detection; Reverse transcription polymerase chain reaction; Strain
• ISSN: 0095-1137; 1098-660X; 1098-5530
• DOI: 10.1128/JCM.02426-06

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.