Phylogenetic analysis of Bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from Aeschynomene species grown in separated geographical regions

• Published in: Applied and Environmental Microbiology Vol. 60; no. 3; pp. 940 - 946
• Main Authors: Wong, F.Y.K, Stackebrandt, E, Ladha, J.K, Fleischman, D.E, Date, R.A, Fuerst, J.A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.1994
• Subjects: ADN; AESCHYNOMENE; ARN RIBOSOMAL; ARN RIBOSOMIAL; BACTERIA; Bacteriology; Biological and medical sciences; BRADYRHIZOBIUM; Bradyrhizobium japonicum; CHIMIOTAXONOMIE; CHLOROPHYLLE; CLOROFILAS; Deoxyribonucleic acid; DNA; FILOGENIA; FOTOSINTESIS; Fundamental and applied biological sciences. Psychology; GENE; GENES; Microbiology; Microorganism-Plant Interactions; Parasitism and symbiosis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; PHOTOSYNTHESE; PHYLOGENIE; Plant physiology and development; QUIMIOTAXONOMIA; RHIZOBIACEAE; Ribonucleic acid; RIBOSOMAS; RIBOSOME; RNA; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Symbiosis; Phylogenetic tree; Bradyrhizobium japonicum; Plant nodule; Aeschynomene; Bacteriochlorophyll a; Amplification; Polymerase chain reaction; Leguminosae; Ribosomal RNA; Dicotyledones; Angiospermae; Nitrogen fixation; 16S-RNA; Bacteria; Spermatophyta; Rhizobiaceae; Photosynthesis
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.60.3.940-946.1994

Nearly complete and short partial 16S rRNA sequences were derived from PCR-amplified ribosomal DNAs of Bradyrhizobium japonicum USDA 136 and USDA 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of Aeschynomene indica and other Aeschynomene species growing in different geographic regions, including India, The Philippines and North America. We confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption spectra of methanol-extracted cells contained a major absorbance peak at 770 nm. Strains isolated on different continents and from different Aeschynomene species were found to be phylogenetically homogeneous and exhibited levels of sequence similarity of more than 99%. The bacteriochlorophyll-synthesizing rhizobia, Bradyrhizobium japonicum, Blastobacter denitrificans, Afipia felis, and Rhodopseudomonas palustris exhibited levels of sequence similarity of 97% or greater and belong to a distinct line of descent within the alpha-2 subdivision of the Proteobacteria. Variable regions between positions 995 and 1045 provide potential target sites for design of a probe that is able to distinguish the photosynthetic rhizobia from closely related taxa