Isolation and identification of bioactive compounds from Antrodia camphorata against ESKAPE pathogens

• Published in: PloS one Vol. 18; no. 10; p. e0293361
• Main Authors: Zhang, Ya-Dong, Liu, Liang-Yan, Wang, Dong, Yuan, Xiao-Long, Zheng, Yuan, Wang, Yi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.10.2023; Public Library of Science (PLoS)
• Subjects: Acetates; Analysis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - pharmacology; Bacteria; Biology and Life Sciences; Drug resistance in microorganisms; Escherichia coli; Esters; Fermentation; Fungi; Health aspects; Humans; Instrument industry; Management; Medicine and Health Sciences; Methicillin; Microbial Sensitivity Tests; Pathogenic microorganisms; Polyporales; Properties; Research and Analysis Methods; Staphylococcus aureus; Tetracycline; Tetracyclines; China; United States; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0293361

Antimicrobial resistance is a major threat to human health globally. Antrodia camphorata was grown in a malt/yeast extract broth liquid medium for 15 days. Then, 4-L fermentation broth was harvested, yielding 7.13 g of the ethyl acetate extract. By tracing the antimicrobial activity, 12.22 mg of the antimicrobial compound was isolated. The structure of 5-methyl-benzo [1,3]-dioxole-4,7-diol (MBBD) was elucidated using NMR and MS data analyses. The antibacterial activity of MBBD was detected through the microbroth dilution method. MBBD exhibited broad-spectrum antibacterial activity. The minimum inhibitory concentration (MIC) range of MBBD for drug-resistant pathogenic bacteria was 64-256 μg/mL, with the lowest MIC observed for Acinetobacter baumannii (64 μg/mL), followed by Pseudomonas aeruginosa (MIC = 128 μg/mL). Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli were also sensitive, with an MIC of 256 μg/mL. The MIC range of MBBD against 10 foodborne pathogens was 12.5-100 μg/mL. Based on the results of this study, MBBD exhibits broad-spectrum antibacterial activity, particularly demonstrating excellent inhibitory effects against A. baumannii. MBBD will be good candidates for new antimicrobial drugs.