Vaccine-specific antibody secreting cells are a robust early marker of LAIV-induced B-cell response in ferrets

• Published in: Vaccine Vol. 30; no. 2; pp. 237 - 246
• Main Authors: Cherukuri, Anu, Servat, Esteban, Woo, Jennifer
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 05.01.2012; Elsevier Limited
• Subjects: Allergy and Immunology; animal models; Animals; antibodies; Antibodies, Viral - blood; Antibody secreting cell; Antibody-Producing Cells - immunology; B-lymphocytes; B-Lymphocytes - immunology; Biomarker of immunogenicity; Biomarkers; blood serum; cell-mediated immunity; Disease Models, Animal; Female; Ferret model of influenza infection; Ferrets; hemagglutination; Hemagglutination Inhibition Tests; Human populations; Human subjects; humans; humoral immunity; Immune correlate; Immune system; Immunogenicity; immunoglobulin A; Immunoglobulin A - immunology; immunoglobulin G; Immunoglobulin G - immunology; Immunologic Memory; Infections; influenza; Influenza A Virus, H1N1 Subtype - immunology; Influenza Vaccines - administration & dosage; Influenza Vaccines - immunology; Live attenuated influenza vaccine; Lung - virology; lungs; Male; mechanism of action; Mustela; Nasal Mucosa - virology; nose; Orthomyxoviridae Infections - prevention & control; seroprevalence; Studies; vaccination; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - immunology; virus replication; viruses; Live attenuated influenza vaccine; Ferret model of influenza infection; Antibody secreting cell; Biomarker of immunogenicity; Immune correlate
• ISSN: 0264-410X; 1873-2518; 1873-2518
• DOI: 10.1016/j.vaccine.2011.11.001

► Antibody secreting cells are an immunogenicity biomarker of influenza vaccination. ► Sensitive correlate of immunogenicity not impacted by prior influenza exposures. ► Seronegative ferret model simulating immunologically naïve pediatric populations. ► Serpositive older ferret model simulating adult human populations. Currently, a robust set of immune correlates for live attenuated influenza vaccine (LAIV) efficacy in humans has not been fully elucidated. The serum hem agglutination inhibition (HAI) assay has been historically used to measure humoral immune responses to injectable inactivated influenza vaccination. However, serum antibody titers do not reliably reflect the complete mechanism of action of LAIV, which is an intranasally delivered vaccine and is expected to induce local mucosal and cellular immune responses in addition to humoral immune responses. Therefore, we designed a study to evaluate potential immune correlates of LAIV vaccination in the ferret animal model of influenza infection. Ferrets were vaccinated with increasing doses of LAIV and four weeks later challenged with a homologous wild-type ( wt) H1N1 strain. Humoral immune responses measured following LAIV vaccination included HAI, serum antibodies and antibody secreting cells (ASC); and the responses were found to correlate with the dose level of LAIV administered in this model. Protection from wt virus challenge was determined by measuring inhibition of wt viral replication in nasal washes and in lung tissue. Results demonstrated that LAIV doses ≥ 5.0 log 10 Plaque Forming Units (PFU) elicited vaccine-specific IgG and IgA ASC frequencies and induced complete protection in the lungs. Further, we developed a novel model utilizing seropositive older ferrets to demonstrate that in the background of previous wt influenza infection LAIV induces a robust vaccine-specific B-cell response even in the absence of serum antibody response, a result that suggests that effector B-cell responses generated by LAIV are not inhibited by prior viral exposure. Finally, we demonstrated that LAIV elicits strain-specific memory B-cell responses that are measurable in a background of wt influenza infections. Taken together, results from these studies identified the antigen-specific ASC frequency as a useful early biomarker of LAIV-induced B-cell immune response.