Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach

• Published in: PloS one Vol. 19; no. 2; p. e0293116
• Main Authors: Raza, Ayesha, Ali, Tayyab, Naeem, Muhammad, Asim, Muhammad, Hussain, Fatma, Li, Zhiye, Nasir, Abdul
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.02.2024; Public Library of Science (PLoS)
• Subjects: Amylases; Antioxidants; Antioxidants - chemistry; Biology and Life Sciences; Chemical properties; Engineering and Technology; Escherichia coli; Health aspects; High performance liquid chromatography; Materia medica, Vegetable; Medicinal plants; Medicine and Health Sciences; Medicine, Botanic; Medicine, Herbal; Nanotechnology; Pharmaceutical research; Physical Sciences; Plant extracts; Plant Extracts - chemistry; Plants, Medicinal - chemistry; Research and Analysis Methods; Suspensions (Chemistry); Swertia - chemistry; Pakistan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0293116

Swertia chirayita is used as a traditional medicinal plant due to its pharmacological activities, including antioxidant, antidiabetic, antimicrobial, and cytotoxic. This study was aimed to evaluate the therapeutic efficacy of newly synthesized nanosuspensions from Swertia chirayita through nanotechnology for enhanced bioactivities. Biochemical characterization was carried out through spectroscopic analyses of HPLC and FTIR. Results revealed that extract contained higher TPCs (569.6 ± 7.8 mg GAE/100 g)) and TFCs (368.5 ± 9.39 mg CE/100 g) than S. chirayita nanosuspension, TPCs (500.6 ± 7.8 500.6 ± 7.8 mg GAE/100 g) and TFCs (229.5± 3.85 mg CE/100 g). Antioxidant activity was evaluated through DPPH scavenging assay, and nanosuspension exhibited a lower DPPH free radical scavenging potential (06 ±3.61) than extract (28.9± 3.85). Anti-dabetic potential was assessed throughα-amylase inhibition and anti-glycation assays. Extract showed higher (41.4%) antiglycation potential than 35.85% nanosuspension and 19.5% α-amylase inhibitory potential than 5% nanosuspension. Biofilm inhibition activity against E. coli was higher in nanosuspension (69.12%) than extract (62.08%). The extract showed high cytotoxicity potential (51.86%) than nanosuspension (33.63%). These nanosuspensions possessed enhanced bioactivities for therapeutic applications could be explored further for the development of new drugs.