Development and validation of a disk solid phase extraction and gas chromatography–mass spectrometry method for MDMA, MDA, HMMA, HMA, MDEA, methamphetamine and amphetamine in sweat

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 852; no. 1; pp. 450 - 458
• Main Authors: De Martinis, Bruno S., Barnes, Allan J., Scheidweiler, Karl B., Huestis, Marilyn A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2007; Elsevier Science
• Subjects: Amphetamine; Amphetamines - analysis; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; GC/MS; General pharmacology; Humans; MDMA; Medical sciences; Methamphetamine; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Sweat - chemistry; Sweat patches; Amphetamine; Sweat patches; GC/MS; Methamphetamine; MDMA; Delivery system; Amphetamine derivatives; Validation; Solid phase extraction; CNS stimulant; Coupled method; Psychotropic; Metamfetamine; Sympathomimetic; Gas chromatography; Dosage form; Development; Amfetamine; Patch; Mass spectrometry; Sweat
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.02.017

We describe the development and validation of a method for the simultaneous quantification of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), 3-hydroxy-4-methoxymethamphetamine (HMMA), 3-hydroxy-4-methoxyamphetamine (HMA), 3,4-methylenedioxyethylamphetamine (MDEA), methamphetamine (MAMP) and amphetamine (AMP) in sweat. Drugs were eluted from PharmChek™ sweat patches with sodium acetate buffer, extracted with disk solid phase extraction and analyzed using GC/MS-EI with selected ion monitoring. Limits of quantification (LOQ) for MDMA, MDEA, MAMP and AMP were 2.5 ng/patch, and 5 ng/patch for MDA, HMA and HMMA. This fully validated procedure was more sensitive than previously published analytical methods and permitted the simultaneous analysis of multiple amphetamine analogs in human sweat.