Diversity of Bacillus-like bacterial community in the sediments of the Bamenwan mangrove wetland in Hainan, China

• Published in: Canadian journal of microbiology Vol. 63; no. 3; pp. 238 - 245
• Main Authors: Liu, Min, Cui, Ying, Chen, Yuqing, Lin, Xiangzhi, Huang, Huiqin, Bao, Shixiang
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.03.2017; Canadian Science Publishing NRC Research Press
• Subjects: 454 pyrosequencing; Alicyclobacillus; Aneurinibacillus; Animals; anti-infective properties; Bacillaceae; Bacillaceae - classification; Bacillaceae - isolation & purification; Bacillaceae - physiology; Bacillus; Bacillus (bacteria); Bacillus okuhidensis; Bacillus-like bacteria; Bacteria; bacterial communities; bactéries de type Bacillus; Biodiversity; China; culture-dependent method; Distribution; diversity; diversité; DNA, Bacterial; DNA, Ribosomal - genetics; Geologic Sediments - microbiology; Halobacillus; mangrove sediment; Marine sediments; Microbial colonies; méthode dépendante de la culture; Natural history; Paenibacillaceae; Paenibacillus; Phylogeny; Planococcaceae; pyroséquençage 454; ribosomal DNA; RNA, Ribosomal, 16S - genetics; Sediment samplers; Sediments; sequence analysis; Sequence Analysis, DNA; Staphylococcus aureus; sédiment de mangrove; Ustilago; Ustilago scitaminea; Virgibacillus; Water Microbiology; Wetlands; China; Hainan China; pyroséquençage 454; diversity; diversité; Bacillus-like bacteria; bactéries de type Bacillus; mangrove sediment; culture-dependent method; 454 pyrosequencing; sédiment de mangrove; méthode dépendante de la culture
• ISSN: 0008-4166; 1480-3275; 1480-3275
• DOI: 10.1139/cjm-2016-0449

Members of the genus Bacillus and related spore-forming genera are ubiquitous. However, Bacillus-like species isolated from marine sediments have attracted less interest than their terrestrial relatives. Here, we investigated the diversity of Bacillus-like bacterial communities in the sediments of the Bamenwan mangrove wetland in Hainan, China, using culture-dependent and culture-independent methods, and present the first report on this subject. We also discovered some potential novel species from the sediment samples. Four families, Bacillaceae (58%), Paenibacillaceae (22%), Alicyclobacillaceae (15%), and Planococcaceae (5%), and 9 genera, Bacillus (42%), Paenibacillus (16%), Halobacillus (13%), Alicyclobacillus (11%), Rummeliibacillus (5%), Cohnella (5%), Tumebacillus (4%), Pontibacillus (3%), and Aneurinibacillus (2%), were identified by pyrosequencing. In contrast, only 4 genera, Bacillus (57%), Paenibacillus (23%), Halobacillus (14%), and Virgibacillus (6%), were detected by the culture-dependent method. In the 16S rDNA sequencing analysis, the isolates HB12036 and HB12037 were closest to Bacillus okuhidensis Kh10-101 T and Paenibacillus xylanilyticus XIL14 T with similarities of 94.8% and 95.9%, respectively, indicating that these were novel species. Bacillus sp. HB12035 and HB12040 exhibited antimicrobial activity against Staphylococcus aureus ATCC 25923, and Bacillus sp. HB12033 exhibited antimicrobial activity against Ustilago scitaminea Syd.