Existence and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei

• Published in: Cell and tissue research Vol. 343; no. 3; pp. 579 - 593
• Main Authors: Tinikul, Yotsawan, Poljaroen, Jaruwan, Nuurai, Parinyaporn, Anuracpreeda, Panat, Chotwiwatthanakun, Charoonroj, Phoungpetchara, Ittipon, Kornthong, Napamanee, Poomtong, Tanes, Hanna, Peter J, Sobhon, Prasert
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.03.2011; Springer; Springer Nature B.V
• Subjects: abdominal ganglia; animal ovaries; Animals; antibodies; Antibodies - metabolism; Biomedical and Life Sciences; Biomedicine; brain; Central nervous system; Central Nervous System - cytology; Central Nervous System - metabolism; Crustaceans; cytoplasm; Decapoda; Female; gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - metabolism; Hormones; Human Genetics; Immunohistochemistry; Litopenaeus vannamei; Molecular Medicine; Nervous system; neurons; Octopodidae; Octopus; oocytes; Ovarian cancer; Ovary - cytology; Ovary - metabolism; Penaeidae - anatomy & histology; Penaeidae - metabolism; Peptides; Peptides - metabolism; Protein Isoforms - metabolism; Proteomics; Regular Article; shrimp; thoracic ganglia; Tissue Distribution; Immunohistochemistry; Crustacea; Ovary; Gonadotropin-releasing hormone; Pacific white shrimp; Central nervous system
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-010-1112-3

We used antibodies against octopus gonadotropin-releasing hormone (octGnRH) and tunicate GnRH (tGnRH-I) in order to investigate the existence and distribution of GnRH-like peptides in the central nervous system (CNS) and in the ovary during various stages of the ovarian cycle of the white shrimp, Litopenaeus vannamei. OctGnRH-immunoreactive and tGnRH-I-immunoreactive neurons and fibers were present in several regions of the supraesophageal ganglion (brain), subesophageal ganglion (SEG), thoracic ganglia, and abdominal ganglia. In the brain, both octGnRH immunoreactivity (ir) and tGnRH-I-ir were detected in neurons of clusters 6, 11, 17, and associated fibers, and the anterior medial protocerebral, posterior medial protocerebral, olfactory, and tegumentary neuropils. In the SEG and thoracic ganglia, octGnRH-immunoreactive and tGnRH-I-immunoreactive neurons and fibers were present in dorsolateral and ventromedial cell clusters and in surrounding fibers. Only immunoreactive fibers were detected in the abdominal ganglia. In the ovary, both octGnRH and tGnRH-I were detected at medium intensity in the cytoplasm of early step oocytes (Oc2) and, at high intensity, in Oc3. Furthermore, octGnRH-ir and tGnRH-I-ir were intense in follicular cells surrounding Oc2 and Oc3. The presence of GnRH-ir in the CNS and ovary indicates that GnRH-like peptides occur in the white shrimp, and that GnRHs are involved in the reproductive process, especially ovarian maturation and the differentiation of oocytes, as reported in other species.