木质素对灵芝菌丝体生长的影响

为了解添加外源木质素对灵芝菌丝体生长的影响,该研究通过在灵芝培养基中添加纤维素、半纤维素和木质素,考察了外源添加木质纤维素主要组分对灵芝菌体生长的影响,发现木质素添加明显促进灵芝菌丝体生长;进一步考察添加不同浓度木质素灵芝菌丝体生长情况以及灵芝产多糖、三萜变化规律。结果表明少量添加木质素对灵芝菌丝体生长有明显促进作用,木质素加量为1.0%时,灵芝菌丝体生长情况最好,生物量比对照提高了56%,同时灵芝胞外多糖、胞内多糖及灵芝胞外三萜、胞内三萜的合成均有明显提高,其中胞内多糖提高43%;4种灵芝菌株发酵产多糖、三萜结果对比分析表明1.0%木质素添加均能提高不同菌株发酵多糖、三萜含量,木质素作为营养...

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Bibliographic Details
Published in农业工程学报 Vol. 33; no. 6; pp. 309 - 314
Main Author 裴海生 孙君社 王民敬 尹腾 张秀清
Format Journal Article
LanguageChinese
Published 农业部规划设计研究院,北京 100125%农业部规划设计研究院,北京,100125%中国农业大学食品科学与营养工程学院,北京,100083 2017
中国农业大学食品科学与营养工程学院,北京 100083
Subjects
三萜
多糖
木质素
液体发酵
灵芝菌
生物量
polysaccharides
灵芝菌
Ganoderma lucidum
生物量
木质素
液体发酵
liquid fermentation
多糖
triterpenes
三萜
lignin
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ISSN1002-6819
DOI10.11975/j.issn.1002-6819.2017.06.040

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Summary:为了解添加外源木质素对灵芝菌丝体生长的影响,该研究通过在灵芝培养基中添加纤维素、半纤维素和木质素,考察了外源添加木质纤维素主要组分对灵芝菌体生长的影响,发现木质素添加明显促进灵芝菌丝体生长;进一步考察添加不同浓度木质素灵芝菌丝体生长情况以及灵芝产多糖、三萜变化规律。结果表明少量添加木质素对灵芝菌丝体生长有明显促进作用,木质素加量为1.0%时,灵芝菌丝体生长情况最好,生物量比对照提高了56%,同时灵芝胞外多糖、胞内多糖及灵芝胞外三萜、胞内三萜的合成均有明显提高,其中胞内多糖提高43%;4种灵芝菌株发酵产多糖、三萜结果对比分析表明1.0%木质素添加均能提高不同菌株发酵多糖、三萜含量,木质素作为营养基质在不同灵芝菌株培养过程中添加均能促进菌丝体活性物质合成。研究结果可为灵芝菌生长代谢研究及人工栽培提供科学依据和参考。
Bibliography:11-2047/S
Pei Haisheng1,2, Sun Junshe2, Wang Minjing2, Yin Teng1, Zhang Xiuqing1 （1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Chinese Academy of Agricultural Engineering, Beijing 1 O0125, China）
lignin; polysaccharides; triterpenes; Ganoderma lucidum; liquid fermentation
Ganoderma lucidum is a typical polypore fungus used for traditional Chinese medical purposes.The active ingredients of Ganoderma lucidum include polysaccharides,triterpene,proteins and ganoderic acid,which can be effective in the treatment of cancer,hypertension and viral infections.Therefore,it has been widely planted and used in human health products.Nowadays the main cultivation methods for Ganoderma lucidum were cut-log cultivation and substitute cultivation.These two methods use abundant wood.So the cultivation of Ganoderma lucidum by using abundant wood could cause destruction to the ecological environment.Ganoderma lucidum liquid fermentation cultivation was no only an i
ISSN:1002-6819
DOI:10.11975/j.issn.1002-6819.2017.06.040