南美白对虾多酚氧化酶的提取及其性质研究
以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3 h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著（P〈0.0001）,方程拟合性好（R-2=0.9762）,可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,V（max）为0.281 A/min。PPO性质研究将为南美白对虾黑...
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| Published in | 广东农业科学 Vol. 44; no. 3; pp. 136 - 142 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海,201306%上海海洋大学水产与生命学院,上海,201306
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
| DOI | 10.16768/j.issn.1004-874X.2017.03.020 |
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| Abstract | 以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3 h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著（P〈0.0001）,方程拟合性好（R-2=0.9762）,可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,V（max）为0.281 A/min。PPO性质研究将为南美白对虾黑变抑制提供理论指导。 |
|---|---|
| AbstractList | TS254; 以南美白对虾为对象,研究了多酚氧化酶(PPO)分离提取工艺及其生化特性.在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析.结果表明:液料比为2:1、提取液体系pH值为8.0、浸提时间为3h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著(P＜0.0001),方程拟合性好(R2=0.9762),可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,Vmax为0.281A/min.PPO性质研究将为南美白对虾黑变抑制提供理论指导. 以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3 h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著（P〈0.0001）,方程拟合性好（R-2=0.9762）,可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,V（max）为0.281 A/min。PPO性质研究将为南美白对虾黑变抑制提供理论指导。 |
| Author | 段秀霞 施文正 邱伟强 方兵 汪之和 江敏 |
| AuthorAffiliation | 上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海201306 上海海洋大学水产与生命学院,上海201306 |
| AuthorAffiliation_xml | – name: 上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海,201306%上海海洋大学水产与生命学院,上海,201306 |
| Author_FL | QIU Wei-qiang JIANG Min DUAN Xiu-xia SHI Wen-zheng WANG Zhi-he FANG Bing |
| Author_FL_xml | – sequence: 1 fullname: DUAN Xiu-xia – sequence: 2 fullname: SHI Wen-zheng – sequence: 3 fullname: QIU Wei-qiang – sequence: 4 fullname: FANG Bing – sequence: 5 fullname: WANG Zhi-he – sequence: 6 fullname: JIANG Min |
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| ClassificationCodes | TS254 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.16768/j.issn.1004-874X.2017.03.020 |
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| DocumentTitleAlternate | Extraction and characteristics of polyphenol oxidase from white shrimp |
| DocumentTitle_FL | Extraction and characteristics of polyphenol oxidase from white shrimp |
| EndPage | 142 |
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| Keywords | 多酚氧化酶(PPO) 反应动力学 响应面优化 南美白对虾 |
| Language | Chinese |
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| Notes | 44-1267/S The optimization of extraction and biological characteristics of polyphenol oxidase （ PPO ） of white shrimp （ Penaeus vannamei ） were studied. The experimental factors and levels were determined by single factor experiments. Response surface methodology was used to explore the effects of solid-liquid ratio, pH and extraction time on PPO activity. The results showed that the optimal extraction conditions were as follows： the solid-liquid ratio was 2 ： 1, pH was 8.0 and the extraction time was 3.0 h. Under the optimal extractions, the PPO activity could reach 94.8 A/rain - mL. Regressive model of extracted PPO was significant （ P 〈 0.0001 ） and better fitting （ R^2=0.9762 ）, it could be used to predict the PPO extraction. The kinetic equation of enzyme reaction was consistent with Michaelis Menten equation. The K,, was 30.322 mmol/L and Vmax was about 0.281 A/min. Study on properties of PPO could provide theoretical guidance for white shrimp anti-melanosis technology. DUAN Xiu-xia1, SHI Wen-zheng1, QIU |
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| PublicationTitle | 广东农业科学 |
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| Publisher | 上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海,201306%上海海洋大学水产与生命学院,上海,201306 |
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| Snippet | 以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3... TS254;... |
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| StartPage | 136 |
| SubjectTerms | 南美白对虾 反应动力学 响应面优化 多酚氧化酶（PPO） |
| Title | 南美白对虾多酚氧化酶的提取及其性质研究 |
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