南美白对虾多酚氧化酶的提取及其性质研究

以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3 h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著（P〈0.0001）,方程拟合性好（R-2=0.9762）,可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,V（max）为0.281 A/min。PPO性质研究将为南美白对虾黑...

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Published in广东农业科学 Vol. 44; no. 3; pp. 136 - 142
Main Author 段秀霞 施文正 邱伟强 方兵 汪之和 江敏
Format Journal Article
LanguageChinese
Published 上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海,201306%上海海洋大学水产与生命学院,上海,201306 2017
Subjects
南美白对虾
反应动力学
响应面优化
多酚氧化酶（PPO）
多酚氧化酶(PPO)
反应动力学
响应面优化
南美白对虾
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ISSN1004-874X
DOI10.16768/j.issn.1004-874X.2017.03.020

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Summary:以南美白对虾为对象,研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试验基础上,采用响应面试验优化了料液比、提取液pH值和浸提时间等因素提取南美白对虾PPO的工艺,并对PPO的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH值为8.0、浸提时间为3 h等条件下,提取的PPO具有最大酶活力为94.8 A/min·mL,所得回归模型显著（P〈0.0001）,方程拟合性好（R-2=0.9762）,可用于预测南美白对虾中PPO的提取;PPO酶促反应动力学方程符合米氏方程,米氏常数Km为30.322 mmol/L,V（max）为0.281 A/min。PPO性质研究将为南美白对虾黑变抑制提供理论指导。
Bibliography:44-1267/S
The optimization of extraction and biological characteristics of polyphenol oxidase （ PPO ） of white shrimp （ Penaeus vannamei ） were studied. The experimental factors and levels were determined by single factor experiments. Response surface methodology was used to explore the effects of solid-liquid ratio, pH and extraction time on PPO activity. The results showed that the optimal extraction conditions were as follows： the solid-liquid ratio was 2 ： 1, pH was 8.0 and the extraction time was 3.0 h. Under the optimal extractions, the PPO activity could reach 94.8 A/rain - mL. Regressive model of extracted PPO was significant （ P 〈 0.0001 ） and better fitting （ R^2=0.9762 ）, it could be used to predict the PPO extraction. The kinetic equation of enzyme reaction was consistent with Michaelis Menten equation. The K,, was 30.322 mmol/L and Vmax was about 0.281 A/min. Study on properties of PPO could provide theoretical guidance for white shrimp anti-melanosis technology.
DUAN Xiu-xia1, SHI Wen-zheng1, QIU
ISSN:1004-874X
DOI:10.16768/j.issn.1004-874X.2017.03.020