云南野生石斛ISSR-PCR分析

为了给石斛属植物种质资源保护和开发利用提供参考,采用ISSR技术从DNA水平探讨9种石斛的亲缘关系和遗传多样性。结果表明,所采用的8条引物共扩增出71个位点,其中多态性位点67个,多态位百分率94.4%,平均每条引物扩增条带数为8.9条;样品间的GS在0.56~0.80,在GS=0.586处将9种石斛植物分为4个大组,并且亲缘关系较近。...

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Published in西南农业学报 Vol. 28; no. 3; pp. 1242 - 1245
Main Author 孔琼 袁盛勇 薛春丽 周锐 周琴 段贤威 张正斌 蒋培春 罗满先
Format Journal Article
LanguageChinese
Published 红河学院生命科学与技术学院,云南蒙自,661199%开远市人民政府发展生物产业办公室,云南开远,661699 2015
Subjects
ISSR-PCR
云南
石斛
Yunnan
ISSR-PCR
石斛
Dendrobium
云南
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2015.03.060

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Summary:为了给石斛属植物种质资源保护和开发利用提供参考,采用ISSR技术从DNA水平探讨9种石斛的亲缘关系和遗传多样性。结果表明,所采用的8条引物共扩增出71个位点,其中多态性位点67个,多态位百分率94.4%,平均每条引物扩增条带数为8.9条;样品间的GS在0.56~0.80,在GS=0.586处将9种石斛植物分为4个大组,并且亲缘关系较近。
Bibliography:In order to provide reference for genetic information in germplasm protection and utilization of Dendrobium, genetic relationship and diversity in nine kinds of Dendrobium were analyzed by eight primers of ISSR. The results showed that 71 loci were detected. There were 67 polymorphie loci among them, and the polymorphic ratio was 94.4 %, and average amplified bands were 8.9 by every primer. The 8e- netic similarity ranged from 0.56 to 0. 80. Nine wild species of Dendrobium could be clustered into four groups at C,S = 0. 586, and these species in lab test had a higher contiguous genetic relationship.
51-1213/S
Dendrobium; ISSR-PCR; Yunnan
KONG Qiong, YUAN Sheng-yong, XUE Chun-li, ZHOU Rui, ZHOU Qin , DUAN Xian-wei , ZHANG Zheng-bin , JIANG Pei-chun , LUO Man-xian（ 1. College of Life and Technology, Honghe University, Yunnan Mengzi 661199, China; 2. Bioindustry Development Office of Kaiyuan City People＇s Government, Yunnan Kaiyuan 661699, China）
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.03.060