超声辅助酶重量法提取树仔菜总膳食纤维

[目的]优化超声辅助酶重量法提取树仔菜总膳食纤维工艺,为后续野生蔬菜膳食纤维研究工作提供技术参考.[方法]以树仔菜为原料,通过单因素试验考察蛋白酶种类、酶添加量、溶液pH、超声时间和超声温度对总膳食纤维提取率的影响,并在此基础上采用正交试验优化酶解工艺.[结果]蛋白酶种类对树仔菜总膳食纤维提取率影响的排序为木瓜蛋白酶〉中性蛋白酶〉碱性蛋白酶〉胰蛋白酶;超声辅助酶重量法提取树仔菜总膳食纤维的最佳工艺条件为：木瓜蛋白酶添加量0.60%、溶液pH 5.0、超声时间50 min、超声温度65℃,对提取率影响程度排序为木瓜蛋白酶添加量〉超声时间〉超声温度〉溶液pH;在优化条件下,树仔菜总膳食纤维提取率为...

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Published in南方农业学报 Vol. 48; no. 6; pp. 1062 - 1067
Main Author 邓爱妮 范琼 周聪 赵敏
Format Journal Article
LanguageChinese
Published 中国热带农业科学院 分析测试中心/海南省热带果蔬产品质量安全重点实验室,海口,571101 2017
Subjects
树仔菜
膳食纤维
超声辅助
酶重量法
ultrasonic-assisted
Sauropus androgynus L. Merr
树仔菜
酶重量法
超声辅助
dietary fiber
enzymetic-gravimetric method
膳食纤维
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Summary:[目的]优化超声辅助酶重量法提取树仔菜总膳食纤维工艺,为后续野生蔬菜膳食纤维研究工作提供技术参考.[方法]以树仔菜为原料,通过单因素试验考察蛋白酶种类、酶添加量、溶液pH、超声时间和超声温度对总膳食纤维提取率的影响,并在此基础上采用正交试验优化酶解工艺.[结果]蛋白酶种类对树仔菜总膳食纤维提取率影响的排序为木瓜蛋白酶〉中性蛋白酶〉碱性蛋白酶〉胰蛋白酶;超声辅助酶重量法提取树仔菜总膳食纤维的最佳工艺条件为：木瓜蛋白酶添加量0.60%、溶液pH 5.0、超声时间50 min、超声温度65℃,对提取率影响程度排序为木瓜蛋白酶添加量〉超声时间〉超声温度〉溶液pH;在优化条件下,树仔菜总膳食纤维提取率为45.0%,变异系数小于5.00%,测定结果与GB 5009.88-2014《食品中膳食纤维的测定》测定结果（44.8%）基本一致.[结论]超声辅助酶重量法提取树仔菜总膳食纤维具有酶解时间短、操作简便等优点,可用于批量样品总膳食纤维的提取.
Bibliography:Sauropus androgynus L. Merr;dietary fiber;enzymetic-gravimetric method;ultrasonic-assisted
45-1381/S
[Objective]The extraction process of total dietary fiber from Sauropus androgynus L. Merr by ultrasonic-as-sisted enzymetic-gravimetric method was optimized, in order to provide a theoretical reference for researching dietary fiber of wild vegetable. [Method]Using S. androgynus L. Merr as materials, the effects of protease types, enzyme amount, so-lution pH, ultrasonic time and ultrasonic temperature on extraction rate of total dietary fiber from S. androgynus L. Merr were investigated by single-factor test. Meanwhile, based on the results of single-factor test, the extraction technology was optimized using orthogonal experiment. [Result]Effects of protease types on extraction efficiency were ranked as follows：papain〉neutral protease〉alkaline protease〉trypsin. The optimal technological parameters for ultrasound-assisted enzyma-tic-gravimetric extraction of S. androgynus L. Merr total dietary fiber were as follow
ISSN:2095-1191
DOI:10.3969/j.issn.2095-1191.2017.06.20