Structure and Function of SV40 Large-T Antigen

The small eukaryotic DNA tumour virus, SV40, has long provided a very useful model for the study of eukaryotic DNA replication and cellular transformation. The viral gene product, large-tumour (large-T) antigen, is essential for the initiation of viral DNA replication and the initiation and maintena...

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Published inPhilosophical transactions of the Royal Society of London. Series B. Biological sciences Vol. 317; no. 1187; pp. 455 - 469
Main Authors Mole, S E, Gannon, J V, Ford, M J, Lane, D P
Format Journal Article
LanguageEnglish
Published England The Royal Society 15.12.1987
Subjects
Antibodies, Monoclonal
Antigens, Polyomavirus Transforming - genetics
beta-Galactosidase - genetics
Cell Transformation, Viral
Cloning, Molecular
DNA Replication
Enzyme-Linked Immunosorbent Assay
Epitopes - analysis
Plasmids
Simian virus 40 - genetics
Simian virus 40 - immunology
Viral Fusion Proteins - analysis
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Summary:The small eukaryotic DNA tumour virus, SV40, has long provided a very useful model for the study of eukaryotic DNA replication and cellular transformation. The viral gene product, large-tumour (large-T) antigen, is essential for the initiation of viral DNA replication and the initiation and maintenance of SV40-virus-mediated cellular transformation. The large-T antigen is a complex multifunctional protein, and to delineate its activity more precisely in viral DNA replication and cellular transformation, small functional domains of the protein have been expressed in Escherichia coli and analysed by using a very extensive library of anti-T monoclonal antibodies.
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ISSN:0962-8436
1471-2970
DOI:10.1098/rstb.1987.0072