Extracellular vesicles derived from MSCs activates dermal papilla cell in vitro and promotes hair follicle conversion from telogen to anagen in mice

• Published in: Scientific reports Vol. 7; no. 1; pp. 15560 - 12
• Main Authors: Rajendran, Ramya Lakshmi, Gangadaran, Prakash, Bak, Soon Sun, Oh, Ji Min, Kalimuthu, Senthilkumar, Lee, Ho Won, Baek, Se Hwan, Zhu, Liya, Sung, Young Kwan, Jeong, Shin Young, Lee, Sang-Woo, Lee, Jaetae, Ahn, Byeong-Cheol
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.11.2017; Nature Publishing Group
• Subjects: 101/28; 13; 13/100; 14; 38; 45/77; 631/136/532/2074; 631/532/2074; 64/60; 96/1; 96/21; AKT protein; Bcl-2 protein; Cell proliferation; Extracellular vesicles; Growth factors; Hair; Hair loss; Humanities and Social Sciences; Insulin-like growth factor I; Mesenchyme; multidisciplinary; Science; Science (multidisciplinary); Secretion; Skin; Stem cells; Vascular endothelial growth factor; Versican; Wnt protein
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-15505-3

Hair loss is a common medical problem. In this study, we investigated the proliferation, migration, and growth factor expression of human dermal papilla (DP) cells in the presence or absence of treatment with mesenchymal stem cell extracellular vesicles (MSC-EVs). In addition, we tested the efficacy of MSC-EV treatment on hair growth in an animal model. MSC-EV treatment increased DP cell proliferation and migration, and elevated the levels of Bcl-2, phosphorylated Akt and ERK. In addition; DP cells treated with MSC-EVs displayed increased expression and secretion of VEGF and IGF-1. Intradermal injection of MSC-EVs into C57BL/6 mice promoted the conversion from telogen to anagen and increased expression of wnt3a, wnt5a and versican was demonstrated. The first time our results suggest that MSC-EVs have a potential to activate DP cells, prolonged survival, induce growth factor activation in vitro , and promotes hair growth in vivo .