Effects of γ-Aminobutyric Acid on Skate Retinal Horizontal Cells: Evidence for an Electrogenic Uptake Mechanism

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 87; no. 22; pp. 8945 - 8949
• Main Authors: Malchow, Robert Paul, Ripps, Harris
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.11.1990; National Acad Sciences
• Subjects: Animals; Bicuculline - pharmacology; Biological and medical sciences; Biological Transport, Active; Cell physiology; Chlorides; Electric Conductivity; Electric current; Electric potential; electrophysiology; Fundamental and applied biological sciences. Psychology; gamma -aminobutyric acid; gamma-Aminobutyric Acid - metabolism; gamma-Aminobutyric Acid - pharmacology; horizontal cells; In Vitro Techniques; Marine; Membrane Potentials; Molecular and cellular biology; Neuroglia; Neurons; neurophysiology; Nipecotic Acids - pharmacology; organic acids; Picrotoxin - pharmacology; Pipettes; Proline - analogs & derivatives; Raja erinacea; Raja ocellata; Receptors; Retina; Retina - cytology; Retina - drug effects; Retina - physiology; retinas; Skates (Fish); Sodium; Sodium - physiology; synapses; Uptake mechanisms; Vision, photoreception; GABA; Reaction mechanism; Vertebrata; Pisces; Vision; Biological activity
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.22.8945

In the retinae of many vertebrates, there are classes of horizontal cell that probably utilize γ-aminobutyric acid (GABA) as a neurotransmitter. As with other amino acid transmitter agents, the postsynaptic action of GABA is thought to be terminated by uptake into neurons and glia surrounding the release site. The present study examined whether an uptake system for GABA could be detected in isolated skate horizontal cells by means of electrophysiological methods. Pressure ejection of GABA onto voltage-clamped horizontal cells produced an inward current that showed no sign of desensitization regardless of the GABA concentration. The dose-response relationship followed simple Michaelis-Menten kinetics, with a half-maximal response elicited at ≈110 μM. Nipecotic acid produced a similar current and reduced the responses to GABA when introduced in the bath solution prior to the GABA pulse. On the other hand, application of 500 μM muscimol or 1 mM baclofen, GABAAand GABABreceptor agonists, respectively, were completely without effect. The GABA-induced current was not blocked by superfusion with 500 μM bicuculline, 500 μM picrotoxin, or 500 μM phaclofen. However, the responses to GABA were abolished when the cells were superfused in Ringer's solution in which choline or lithium had been substituted for sodium, and were reduced when the extracellular chloride concentration was decreased from 266 mM to 16 mM. Current-voltage data showed a maximal response to GABA when the cells were held at or below their resting potential. At more depolarized levels, the inward current became progressively smaller until, near +50 mV, it could no longer be detected; over the range tested (-90 to +50 mV), the response never reversed into an outward current. These findings suggest that the GABA-induced currents in skate horizontal cells are mediated by an electrogenic uptake mechanism.