CD62 and Endothelial Cell-Leukocyte Adhesion Molecule 1 (ELAM-1) Recognize the Same Carbohydrate Ligand, Sialyl-Lewis x

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 14; pp. 6224 - 6228
• Main Authors: Polley, Margaret J., Phillips, M. Laurie, Wayner, Elizabeth, Nudelman, Edward, Singhal, Anil K., Hakomori, Sen-Itiroh, Paulson, James C.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15.07.1991; National Acad Sciences
• Subjects: Animals; Antibodies, Monoclonal; Antigens, CD - immunology; Biological and medical sciences; Blood Platelets - immunology; Blood Platelets - physiology; Carbohydrate Conformation; Carbohydrate Sequence; Carbohydrates; Cell Adhesion; Cell Adhesion Molecules - immunology; Cell interactions, adhesion; Cell Line; Cells, Cultured; E-Selectin; Endothelial cells; Endothelium, Vascular - immunology; Endothelium, Vascular - physiology; Fundamental and applied biological sciences. Psychology; Glycolipids; Hematopoietic stem cells; Humans; Lewis X Antigen - immunology; Ligands; Liposomes; Molecular and cellular biology; Molecular Sequence Data; Neutrophils; Neutrophils - immunology; Neutrophils - physiology; Oligosaccharides; P-Selectin; Platelet Activation; Platelet Membrane Glycoproteins - immunology; Platelets; Receptors, Immunologic - immunology; Endothelial cell; Sialic acid; Platelet; Cell line; Leukocyte; Cell adhesion molecule; Cell cell interaction; Carbohydrate; Glycoproteins; Adhesion
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.14.6224

The leukocyte receptor CD62, which is expressed on activated platelets and endothelial cells, is shown to mediate cell adhesion by binding a sialylated carbohydrate structure, sialyl-Lewis x, found on neutrophils, monocytes, and tumor cells. This structure has previously been identified as the ligand for another member of the LEC-CAM family of cell adhesion molecules, endothelial cell-leukocyte adhesion molecule 1, which also binds neutrophils and monocytes. The results demonstrate that although the two LEC-CAMs differ in their biological activities by their distribution and mode of expression, they are capable of mediating cell adhesion by recognition of the same carbohydrate ligand.