Effects of Source Water Quality on Chlorine Inactivation of Adenovirus, Coxsackievirus, Echovirus, and Murine Norovirus

• Published in: Applied and Environmental Microbiology Vol. 76; no. 15; pp. 5159 - 5164
• Main Authors: Kahler, Amy M, Cromeans, Theresa L, Roberts, Jacquelin M, Hill, Vincent R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2010; American Society for Microbiology (ASM)
• Subjects: Adenoviridae; Adenovirus; Adenoviruses, Human - drug effects; Biological and medical sciences; Chlorine; Chlorine - pharmacology; Coxsackievirus; coxsackievirus B5; Disinfectants - pharmacology; Disinfection & disinfectants; Echovirus; Enterovirus; Enterovirus - drug effects; Enterovirus B, Human - drug effects; Fundamental and applied biological sciences. Psychology; Human adenovirus 2; Hydrogen-Ion Concentration; Microbial Viability - drug effects; Microbiology; Norovirus; Norovirus - drug effects; Picornaviridae; Public Health Microbiology; Viral Load; Virus Inactivation; Viruses; Water Microbiology; Water quality; Water treatment; Coxsackievirus; Adenoviridae; Picornaviridae; Chlorine; Rodentia; Enterovirus; Inactivation; Virus; Echovirus; Vertebrata; Mammalia; Mouse; Water quality
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/aem.00869-10

More information is needed on the disinfection efficacy of chlorine for viruses in source water. In this study, chlorine disinfection efficacy was investigated for USEPA Contaminant Candidate List viruses coxsackievirus B5 (CVB5), echovirus 1 (E1), murine norovirus (MNV), and human adenovirus 2 (HAdV2) in one untreated groundwater source and two partially treated surface waters. Disinfection experiments using pH 7 and 8 source water were carried out in duplicate, using 0.2 and 1 mg/liter free chlorine at 5 and 15°C. The efficiency factor Hom (EFH) model was used to calculate disinfectant concentration x contact time (CT) values (mg·min/liter) required to achieve 2-, 3-, and 4-log₁₀ reductions in viral titers. In all water types, chlorine disinfection was most effective for MNV, with 3-log₁₀ CT values at 5°C ranging from [less-than or equal to]0.020 to 0.034. Chlorine disinfection was least effective for CVB5 in all water types, with 3-log₁₀ CT values at 5°C ranging from 2.3 to 7.9. Overall, disinfection proceeded faster at 15°C and pH 7 for all water types. Inactivation of the study viruses was significantly different between water types, but no single source water had consistently different inactivation rates than another. CT values for CVB5 in one type of source water exceeded the recommended CT values set forth by USEPA's Guidance Manual for Compliance with the Filtration and Disinfection Requirements for Public Water Systems using Surface Water Sources. The results of this study demonstrate that water quality plays a substantial role in the inactivation of viruses and should be considered when developing chlorination plans.