Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid

• Published in: Journal of experimental botany Vol. 57; no. 10; pp. 2193 - 2201
• Main Authors: Eichhorn, Heiko, Klinghammer, Michaela, Becht, Philip, Tenhaken, Raimund
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.01.2006; Oxford Publishing Limited (England)
• Subjects: ABC transporter gene; ABC transporters; amino acid sequences; Arabidopsis thaliana; ATP binding cassette transporters; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - physiology; biochemical pathways; Biological and medical sciences; Cell death; cell suspension culture; Complementary DNA; Cyclopentanes; Fundamental and applied biological sciences. Psychology; gene expression; gene expression regulation; Gene Expression Regulation, Plant; Gene induction; Genes; Genes, Plant; Genes. Genome; Glycine max; Glycine max - genetics; Glycine max - physiology; hypersensitive response; messenger RNA; methyl jasmonate; Molecular and cellular biology; Molecular genetics; molecular sequence data; nucleic acid hybridization; nucleotide sequences; Oxylipins; Pathogens; Plant cells; plant pathogenic bacteria; Plants; pleiotropic drug resistance-type transporters; protein structure; Pseudomonas syringae; Pseudomonas syringae pv. glycinea; Research Papers; RNA; Salicylates; salicylic acid; soybean; Soybeans; Subtractive suppression hybridization; transcriptional activation; transporters; soybean; salicylic acid; Subtractive suppression hybridization; ABC transporter gene; Glycine max; Salicylic acid; Hybridization; Arabidopsis thaliana; Leguminosae; Complementary DNA; Gene; Cruciferae; Dicotyledones; Aminoacid; Angiospermae; Spermatophyta; ATP
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erj179

This paper reports on the identification and characterization of a new ATP-binding cassette (ABC) transporter which was identified as a salicylic acid-induced gene from soybean (Glycine max cv. Williams 82) in a subtractive suppression hybridization approach. A fragment of an ABC-transporter gene was used to isolate a full-length cDNA clone for this gene with a length of 4750 bp. The encoded protein has a length of 1447 amino acids and is composed of two similar repeat units typical of full-size ABC transporters. The sequence displays a close relationship to plant pleiotropic drug resistance (PDR)-type transporters and, on a homology basis, clusters together with the Arabidopsis thaliana PDR12 gene, suggesting GmPDR12 as a name for the gene isolated from soybean. GmPDR12 is rapidly responsive to salicylic acid and methyl jasmonate. The mRNA starts to accumulate 30 min after the addition of the signalling compounds. Salicylic acid is required for the execution of the hypersensitive reaction in soybean cell suspension cultures inoculated with Pseudomonas syringae pv. glycinea. It has been demonstrated previously that salicylic acid can be substituted by a variety of functional analogues of salicylic acid. All of these compounds lead to a strong and rapid transcriptional activation of GmPDR12, suggesting a shared signalling pathway.