Directed Network Wiring Identifies a Key Protein Interaction in Embryonic Stem Cell Differentiation
Cell signaling depends on dynamic protein-protein interaction (PPI) networks, often assembled through modular domains each interacting with multiple peptide motifs. This complexity raises a conceptual challenge, namely to define whether a particular cellular response requires assembly of the complet...
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Published in | Molecular cell Vol. 54; no. 6; pp. 1034 - 1041 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
19.06.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Cell signaling depends on dynamic protein-protein interaction (PPI) networks, often assembled through modular domains each interacting with multiple peptide motifs. This complexity raises a conceptual challenge, namely to define whether a particular cellular response requires assembly of the complete PPI network of interest or can be driven by a specific interaction. To address this issue, we designed variants of the Grb2 SH2 domain (“pY-clamps”) whose specificity is highly biased toward a single phosphotyrosine (pY) motif among many potential pYXNX Grb2-binding sites. Surprisingly, directing Grb2 predominantly to a single pY site of the Ptpn11/Shp2 phosphatase, but not other sites tested, was sufficient for differentiation of the essential primitive endoderm lineage from embryonic stem cells. Our data suggest that discrete connections within complex PPI networks can underpin regulation of particular biological events. We propose that this directed wiring approach will be of general utility in functionally annotating specific PPIs.
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•Defining roles of individual interactions in complex networks is challenging•Protein design tailors Grb2 SH2 into “pY-clamps” specific for single pY-ligands•Rewiring the Grb2 PPI network using synthetic Grb2 comprising pY-clamps•Identified Ptpn11 pY580 as a key Grb2 SH2 ligand in stem cell fate specification
Interactions of Grb2 with multiple ligands within a signaling network present a challenge in defining the mechanism underlying a cellular response. Here Yasui et al. engineer Grb2 molecules whose SH2 specificity is directed to a single ligand and find that the PTPN11-Grb2 pathway is responsible for embryonic stem cell differentiation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally. Deceased. |
ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/j.molcel.2014.05.002 |