Musca domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells through a Mitochondria-Mediated Reactive Oxygen Species Way

• Published in: Biological & pharmaceutical bulletin Vol. 33; no. 8; pp. 1274 - 1278
• Main Authors: Zhao, Qi, Cao, Xiaohong, Zeng, Bin, Wang, Chunling, Yan, Le, Xu, Chengjian
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 2010; Japan Science and Technology Agency
• Subjects: Animals; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; antitumor; apoptosis; Apoptosis - drug effects; Apoptosis Regulatory Proteins - biosynthesis; Blotting, Western; Cell Culture Techniques; Cell Cycle - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Flow Cytometry; Houseflies - chemistry; Humans; Larva - chemistry; lectin; Lectins - isolation & purification; Lectins - pharmacology; Membrane Potential, Mitochondrial - drug effects; Microscopy, Fluorescence; Mitochondria - drug effects; Mitochondria - metabolism; mitochondrial path; Musca domestica; Musca domestica larva; Reactive Oxygen Species - metabolism
• ISSN: 0918-6158; 1347-5215; 1347-5215
• DOI: 10.1248/bpb.33.1274

A lectin from Musca domestica larva was purified by affinity chromatography on a glactose-Sepharose 4B column. Musca domestica larva lectin (MLL) inhibited the growth of BEL-7402 cells in a time and concentration-dependent way. The results of Hoechst 33258 staining indicated that MLL induce BEL-7402 cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that MLL treatment abrogated glutathione antioxidant system and induced mitochondria to generate reactive oxygen species (ROS) accumulation, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of Bax, the downregulation of Bcl-2, the cytochrome c release and the activation of the caspases pathways.