云南玉溪烟区KRK26在烘烤中的脂氧合酶比活力及其与质体色素降解的相关性

用分光光度法和相关性分析方法研究了云南玉溪烟区津巴布韦烤烟品种KRK26成熟中部叶片在3段式烘烤中的脂氧合酶比活力及其与质体色素降解速率的相关性。结果表明,KRK26脂氧合酶比活力在烘烤中表现为,低—高—低的趋势,在烘烤不同阶段的差异达到极显著水平,最高峰出现在变黄中期;脂氧合酶比活力与叶绿素a和叶绿素b的相对降解速率呈负相关,但与总叶绿素和类胡萝卜素的相对降解速率呈正相关,相关系数分别为0.014和0.644。因此,对于KRK26在烘烤中的质体色素降解,脂氧合酶可能主要作用于类胡萝卜素而不是叶绿素。本研究结果可为云南烟区KRK26香气品质的评价与调控及烘烤参数的优化提供参考。...

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Bibliographic Details
Published in西南农业学报 Vol. 24; no. 5; pp. 1708 - 1713
Main Author 赵昶灵 杨焕文 邓建华 毛自朝 王金桐 杨龙林
Format Journal Article
LanguageChinese
Published 云南农业大学农学与生物技术学院,云南昆明,650201%云南农业大学烟草学院,云南昆明,650201%云南省烟草农业科学研究院,云南玉溪,653100 2011
Subjects
津巴布韦烤烟品种KRK26
烘烤
相关性
脂氧合酶比活力
质体色素降解
脂氧合酶比活力
烘烤
相关性
津巴布韦烤烟品种KRK26
质体色素降解
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2011.05.019

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Summary:用分光光度法和相关性分析方法研究了云南玉溪烟区津巴布韦烤烟品种KRK26成熟中部叶片在3段式烘烤中的脂氧合酶比活力及其与质体色素降解速率的相关性。结果表明,KRK26脂氧合酶比活力在烘烤中表现为,低—高—低的趋势,在烘烤不同阶段的差异达到极显著水平,最高峰出现在变黄中期;脂氧合酶比活力与叶绿素a和叶绿素b的相对降解速率呈负相关,但与总叶绿素和类胡萝卜素的相对降解速率呈正相关,相关系数分别为0.014和0.644。因此,对于KRK26在烘烤中的质体色素降解,脂氧合酶可能主要作用于类胡萝卜素而不是叶绿素。本研究结果可为云南烟区KRK26香气品质的评价与调控及烘烤参数的优化提供参考。
Bibliography:Zimbabwe tobacco variety KRK26; Flue-curing; Lipoxygenase specific activity; Plastid pigment degradation; Correlation
51-1213/S
The lipoxygenase（LOX） specific activities of the mature middle leaves of the Zimbabwe tobacco variety KRK26 from Yuxi tobacco-growing area of Yunnan province during three phase flue-curing and its correlations with the plastid pigment degradation rate were studied based on spectrophotometry and correlation analysis.The results indicated that： during flue-curing,the LOX specific activity of KRK26 displayed a general tendency of low-high-low,the difference between different flue-curing stages was extremely significant,and the climax emerged at the middle period of the yellowing.The LOX specific activity was negatively correlated to the relative degradation rates of Chlorophyll（Chl） a and b,but positively correlated to those of carotinoid（Car）（r=0.644）and Chl（a ＋ b）（r=0.014）.Thus,as to the plastid pigment degradation of KRK26 during flue-curing,LOX appears to function mainly not on the Ch
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2011.05.019