光照强度对褐牙鲆血浆皮质醇、渗透压和生化指标的影响

将褐牙鲆(Paralichthys olivaceus)在0、10、100、1 000 lx等4个不同光照强度下分别处理30 min和7d,探讨不同光照强度对褐牙鲆血浆皮质醇、渗透压、血浆中总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(TC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)等指标的影响.结果显示,改变光照强度30 min会影响皮质醇的变化,10 lx组皮质醇水平最低,且随着时间延长至7d,整体水平皆有所降低但趋势保持一致.不同光照强度处理对褐牙鲆血浆渗透压及血浆中TP、ALB含量无显著影响;不同光照强度处理后30 min,100 lx处理TG含...

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Published in广东农业科学 Vol. 41; no. 11; pp. 131 - 135
Main Author 王帅 陈阿琴 吕为群
Format Journal Article
LanguageChinese
Published 上海海洋大学水产与生命学院,上海,201306 2014
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ISSN1004-874X

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Summary:将褐牙鲆(Paralichthys olivaceus)在0、10、100、1 000 lx等4个不同光照强度下分别处理30 min和7d,探讨不同光照强度对褐牙鲆血浆皮质醇、渗透压、血浆中总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(TC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)等指标的影响.结果显示,改变光照强度30 min会影响皮质醇的变化,10 lx组皮质醇水平最低,且随着时间延长至7d,整体水平皆有所降低但趋势保持一致.不同光照强度处理对褐牙鲆血浆渗透压及血浆中TP、ALB含量无显著影响;不同光照强度处理后30 min,100 lx处理TG含量最低且与0 lx处理差异显著,但在处理后7 d 100 lx处理TG含量最高;TC含量变化与TG相似,即随着处理时间的延长,100 lx处理TC含量呈升高的趋势,但10 lx处理无明显变化.不同光照强度下处理后30 min,10 lx处理AST、ALT活性最低,且与1 000 lx处理差异显著,处理后7d,仍维持此变化趋势;在短期光强刺激下,各处理ALP活性无显著差异,但在长期光强刺激下,0 lx处理显著低于10 lx处理和100 lx处理.结果表明,褐牙鲆适宜的光强范围为10 lx左右,褐牙鲆通过积极的生理调节以适应不同的光照强度变化,这对其适应环境变化具有重要的意义.
Bibliography:44-1267/S
WANG Shuai, CHEN A-qin, LYU Wei-qun (College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China)
In order to determine the effects of light intensity on the plasma cortisol, osmolality and biochemical indices including the contents of total protein(TP), albumin(ALB), triglycerides(TG), total cholesterol(TC), aspertate aminotransferase (AST), glutamic-pyruvic transaminase (ALT) and the activity of alkaline phosphatase (ALP) of Japanese flounder (Paralichthys olivaceus), 72 flounders were treated with four light intensity(0 lx, 10 lx, 100 lx, and 1 000 lx) for 30 min or 7 days. The results showed that, after treating 30 min, cortisol changed, and it reached the lowest in 10 lx group. As time extended to 7 d, cortisol decreased in all light intensity, but the trend was consistent. Light intensity had no significant effect on the contents of osmolality, TP and ALB. The content of TG in 100 lx group treated with 30 min was the lowest and significantly lower than those in 10 lx
ISSN:1004-874X