氮胁迫条件下稻瘟病菌分泌蛋白与水稻悬浮细胞互作中“活性氧”变化分析

以CH-63和TH-16 2个致病性差异显著的稻瘟病菌株为材料,在氮胁迫培养条件下进行液体培养,经培养2 d后,采用铵盐沉淀及透析纯化提取稻瘟病菌培养滤液中的致病性分泌蛋白。以24个IRRI水稻抗稻瘟病单基因系和普感品种丽江新团黑谷（LTH）的成熟种胚为材料,诱导愈伤组织并构建水稻悬浮细胞系。以构建成功的感病品种LTH和抗病品种IRBL-9的水稻悬浮细胞系为材料,接种稻瘟病菌致病性分泌蛋白并对活性氧浓度变化进行检测。结果表明,感病与抗病品种在接种前12 h均出现活性氧迸发,且均出现2个峰值。比较稻瘟病菌株TH-16和CH-63分泌蛋白接种IRBL-9与LTH后H2O2浓度变化特征,发现分泌蛋白...

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Bibliographic Details
Published in西南农业学报 Vol. 24; no. 6; pp. 2164 - 2171
Main Author 周晓罡 侯思名 苏源 陶南 丁玉梅 姚春馨 孙茂林 张绍松
Format Journal Article
LanguageChinese
Published 云南省农业科学院生物技术与种质资源研究所,云南昆明650223 2011
云南省农业生物技术重点实验室,云南昆明650223%昆明大学生命科学与技术学院,云南昆明,650031
Subjects
分泌蛋白
悬浮细胞系
氮胁迫
活性氧
致病性
致病性
分泌蛋白
悬浮细胞系
活性氧
氮胁迫
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2011.06.029

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Summary:以CH-63和TH-16 2个致病性差异显著的稻瘟病菌株为材料,在氮胁迫培养条件下进行液体培养,经培养2 d后,采用铵盐沉淀及透析纯化提取稻瘟病菌培养滤液中的致病性分泌蛋白。以24个IRRI水稻抗稻瘟病单基因系和普感品种丽江新团黑谷（LTH）的成熟种胚为材料,诱导愈伤组织并构建水稻悬浮细胞系。以构建成功的感病品种LTH和抗病品种IRBL-9的水稻悬浮细胞系为材料,接种稻瘟病菌致病性分泌蛋白并对活性氧浓度变化进行检测。结果表明,感病与抗病品种在接种前12 h均出现活性氧迸发,且均出现2个峰值。比较稻瘟病菌株TH-16和CH-63分泌蛋白接种IRBL-9与LTH后H2O2浓度变化特征,发现分泌蛋白致病性强弱与H2O2浓度变化呈正相关,CH-63产生的强致病性分泌蛋白引起活性氧浓度的快速增加。TH-16产生的弱致病性分泌蛋白接种IRBL-9与LTH后活性氧浓度则增加不明显。上述研究对于深入了解稻瘟病菌与水稻之间的相互识别、信号传导和应答过程,阐明水稻对稻瘟病菌的抗性机制、水稻与稻瘟病菌互作的分子机理具有重要意义。
Bibliography:The rice blast fungal strains CH-63 and TH-16 with 2 different pathogenic factors were cultivated in a liquid culture medium under nitrogen starvation.After 2 d of cultivation,the protein secreted by the fungal strains was extracted by ammonium sulfate sedimentation and purified by dialysis.Mature embryos of the above mentioned 24 monogenic rice lines and universally susceptible japonica rice variety Lijiangxintuanheigu（LTH） were used as materials to induce callus in inducing medium I and II;the callus-inducing capability varied among the different rice genotypes,and the callus induction rate ranged from 15 % to 98 %.When the callus was cultivated on a subculture medium for some days,the well-grown calli were isolated to develop cell suspension lines of rice.The shape of the suspension cell was observed during the process of cultivation.At the start of the culture,the cells were abnormal in shape and were long,and their vacuole and cytoplasm were big and rarefied,respectively.At the metaphase of culture,the c
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2011.06.029