22份枸杞属种质的RAPD分析

对22份枸杞属种质材料进行RAPD分析。研究结果表明，32个引物在22份种质中共扩增出了204条带、其中162条为多态性条带，多态性条带的比例为79．41％。UPGMA聚类分析结果表明，RAPD分子标记的聚类结果与枸杞属形态分类基本相似，能将种间亲缘关系划分出，但是对变种与种问关系的区分不佳。...

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Bibliographic Details
Published in广东农业科学 Vol. 39; no. 5; pp. 112 - 113
Main Author 张波 李敦 戴国礼 曹有龙 赵建华
Format Journal Article
LanguageChinese
Published 宁夏大学生命科学学院,宁夏银川750002%平罗县农牧局,宁夏平罗,753400%国家枸杞工程技术研究中心,宁夏银川,750002 2012
国家枸杞工程技术研究中心,宁夏银川750002
Subjects
RAPD
枸杞
种质
种质
RAPD
枸杞
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Summary:对22份枸杞属种质材料进行RAPD分析。研究结果表明，32个引物在22份种质中共扩增出了204条带、其中162条为多态性条带，多态性条带的比例为79．41％。UPGMA聚类分析结果表明，RAPD分子标记的聚类结果与枸杞属形态分类基本相似，能将种间亲缘关系划分出，但是对变种与种问关系的区分不佳。
Bibliography:44-1267/S
wolfberry;germplasms;RAPD
The genomic DNA of 22 wolfberry germplasms were tested with RAPD using 32 arbitrary lO-mer primers which were screened from 100 primers.The 162 polymorphic bands of 204 RAPD bands were obtained with thepercentage of polymorphic bands 79.41%. UPGMA cluster analysis based on RAPD markers data.The result showed that the RAPD approach was efficient in generating accurate information on genetic background, relationship and evolution of wo｜iberry gcrmplasms.
ZHANG Bo, LI Dun, DAI Guo-li, CAO You-long, ZHAO Jian-hua （1.National Wolfoerry Engineering and Technology Research Center, Yinchnan 750002, China; 2. College of Life Sciences Ningxia, Ningxia University, Yinchuan 750002, China; 3. Pingluo Agriculture and A nirnd Husbandry, Pingluo 753400, China）
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2012.05.039