微波辅助提取绿茶茶多酚及纯化工艺研究
为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚。研究确定茶多酚提取工艺流程为：微波功率在600W,料液比为1∶25,浸提时间为7min,浸提次数为2次效果最好,其浸提率达84.73%。采用中空纤维膜超滤,膜孔径在0.0015～0.02μm,操作压力0.10～0.12 Mpa。选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46 mg/mL,上样流速1.4 mL/min,溶液pH值3.0。上样体积600 mL,洗脱剂80%（体积比）食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL。低压下抽...
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| Published in | 西南农业学报 Vol. 25; no. 3; pp. 1074 - 1079 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
重庆市农业科学院茶叶研究所,重庆永川,402160%重庆市农业科学院农产品贮藏与加工研究所,重庆,402139%重庆市农业科学院农业科技信息中心,重庆,402139
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.3969/j.issn.1001-4829.2012.03.069 |
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| Abstract | 为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚。研究确定茶多酚提取工艺流程为：微波功率在600W,料液比为1∶25,浸提时间为7min,浸提次数为2次效果最好,其浸提率达84.73%。采用中空纤维膜超滤,膜孔径在0.0015～0.02μm,操作压力0.10～0.12 Mpa。选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46 mg/mL,上样流速1.4 mL/min,溶液pH值3.0。上样体积600 mL,洗脱剂80%（体积比）食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL。低压下抽真空干燥,得纯度高于95%、咖啡因含量低于1%的茶多酚样品。 |
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| AbstractList | 为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚。研究确定茶多酚提取工艺流程为：微波功率在600W,料液比为1∶25,浸提时间为7min,浸提次数为2次效果最好,其浸提率达84.73%。采用中空纤维膜超滤,膜孔径在0.0015～0.02μm,操作压力0.10～0.12 Mpa。选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46 mg/mL,上样流速1.4 mL/min,溶液pH值3.0。上样体积600 mL,洗脱剂80%（体积比）食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL。低压下抽真空干燥,得纯度高于95%、咖啡因含量低于1%的茶多酚样品。 S571.1; 为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚.研究确定茶多酚提取工艺流程为:微波功率在600W,料液比为1∶25,浸提时间为7min,浸提数为2次效果最好,其浸提率达84.73％.采用中空纤维膜超滤,膜孔径在0.0015～0.02 μm,操作压力0.10 ～0.12 Mpa.选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46mg/L,上样流速1.4 mL/min,溶液pH值3.0.上样体积600mL,洗脱剂80％(体积比)食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL.低压下抽真空干燥,得纯度高于95％、咖啡因含量低于1％的茶多酚样品. |
| Author | 袁林颖 高飞虎 钟应富 邬秀宏 邓敏 张莹 敬廷桃 |
| AuthorAffiliation | 重庆市农业科学院茶叶研究所,重庆永川402160 重庆市农业科学院农产品贮藏与加工研究所,重庆402139 重庆市农业科学院农业科技信息中心,重庆402139 |
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| Author_FL | JING Ting-tao ZHONG Ying-fu ZHANG Ying YUAN Lin-yin WU Xiu-hong DENG Min GAO Fei-hu |
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| DocumentTitleAlternate | Study on Microwave-assisted Extraction Polyphenolics and Purification Technology from Green Tea |
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| Keywords | 纯化 茶多酚 提取 分离 |
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| Notes | Polyphenolics; Extraction ; Separate; Purification 51-1213/S YUAN Lin-ying , GAO Fei-hu , ZHONG ring-fu , WU Xiu-hong , DENG Min , ZHANG Ying , JING Tirtg-tao（ 1. Institute of Tea, Chongqing Academy of Agricultural Sciences, Chongqing Yongehuan 402160, China;2. Institute of Agricultural Product Storage and Processing, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China ;3. Agricultural Science and Technology In- formation Center,Chongqing Academy of Agricultural Sciences, Chongqing 401329,China） In order to raise the extraction rate and pureness of polyphenolics, reduce using level of organic solvent and choose 80-mu green tea, taking water as extracting agent, polyphenolics was microwave-assisted extracted by and macmpemus resin. The results showed that when the technological process of polypbenolies extraction was 600 w microwave power, 1：25 feed liquid,7 rain digestion and 2 times, the digestion rate was up to 84.73 %. By ultrafiltration of the hollow fiber membrane, the membrane aperture was |
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| SubjectTerms | 分离 提取 纯化 茶多酚 |
| Title | 微波辅助提取绿茶茶多酚及纯化工艺研究 |
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