微波辅助提取绿茶茶多酚及纯化工艺研究

为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚。研究确定茶多酚提取工艺流程为：微波功率在600W,料液比为1∶25,浸提时间为7min,浸提次数为2次效果最好,其浸提率达84.73%。采用中空纤维膜超滤,膜孔径在0.0015～0.02μm,操作压力0.10～0.12 Mpa。选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46 mg/mL,上样流速1.4 mL/min,溶液pH值3.0。上样体积600 mL,洗脱剂80%（体积比）食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL。低压下抽...

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Published in西南农业学报 Vol. 25; no. 3; pp. 1074 - 1079
Main Author 袁林颖 高飞虎 钟应富 邬秀宏 邓敏 张莹 敬廷桃
Format Journal Article
LanguageChinese
Published 重庆市农业科学院茶叶研究所,重庆永川,402160%重庆市农业科学院农产品贮藏与加工研究所,重庆,402139%重庆市农业科学院农业科技信息中心,重庆,402139 2012
Subjects
分离
提取
纯化
茶多酚
纯化
茶多酚
提取
分离
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2012.03.069

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Summary:为提高茶多酚提取率及纯度,减少有机溶剂用量,选择粉碎粒度为80目的绿茶粉,利用水为提取剂,微波辅助提取,超滤分离、大孔树脂纯化提取茶多酚。研究确定茶多酚提取工艺流程为：微波功率在600W,料液比为1∶25,浸提时间为7min,浸提次数为2次效果最好,其浸提率达84.73%。采用中空纤维膜超滤,膜孔径在0.0015～0.02μm,操作压力0.10～0.12 Mpa。选择4号大孔吸附树脂分离纯化茶多酚,上样浓度0.46 mg/mL,上样流速1.4 mL/min,溶液pH值3.0。上样体积600 mL,洗脱剂80%（体积比）食用乙醇,洗脱流速为2.0 mL/min,洗脱剂用量为520 mL。低压下抽真空干燥,得纯度高于95%、咖啡因含量低于1%的茶多酚样品。
Bibliography:Polyphenolics; Extraction ; Separate; Purification
51-1213/S
YUAN Lin-ying , GAO Fei-hu , ZHONG ring-fu , WU Xiu-hong , DENG Min , ZHANG Ying , JING Tirtg-tao（ 1. Institute of Tea, Chongqing Academy of Agricultural Sciences, Chongqing Yongehuan 402160, China;2. Institute of Agricultural Product Storage and Processing, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China ;3. Agricultural Science and Technology In- formation Center,Chongqing Academy of Agricultural Sciences, Chongqing 401329,China）
In order to raise the extraction rate and pureness of polyphenolics, reduce using level of organic solvent and choose 80-mu green tea, taking water as extracting agent, polyphenolics was microwave-assisted extracted by and macmpemus resin. The results showed that when the technological process of polypbenolies extraction was 600 w microwave power, 1：25 feed liquid,7 rain digestion and 2 times, the digestion rate was up to 84.73 %. By ultrafiltration of the hollow fiber membrane, the membrane aperture was
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.03.069