普安银鲫胚胎发育早期的总脂与脂肪酸变化

为了探明普安银鲫在胚胎发育过程中的能量来源、组成变化和利用模式,采用生物化学方法测定普安银鲫的成熟卵和8个胚胎发育时期的总脂与脂肪酸组成变化。结果表明：①普安银鲫胚胎中成熟卵和胚胎不同发育时期的总脂含量随着胚胎的不断发育而降低,成熟卵与受精卵相比,总脂稍有下降,由其干重的18.25%降至17.94%,但差异不显著（P〉0.05）,成熟卵随受精后胚胎发育的推进,其总脂变化呈逐渐降低趋势,在成熟卵和早期胚胎中的主要脂肪酸为C16：0,C18：0,C18：1n-9和C18：2n-6;与成熟卵相比,普安银鲫受精卵的饱和脂肪酸（SFA）与高度不饱和脂肪酸（HUFA）下降,单不饱和脂肪酸（MUFA）升高。...

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Published in西南农业学报 Vol. 25; no. 5; pp. 1925 - 1928
Main Author 牟洪民 马珊 姚俊杰 安苗 王艳艳 梁正其
Format Journal Article
LanguageChinese
Published 贵州大学动物科学学院水产科学系,贵州贵阳,550025 2012
Subjects
总脂
普安银鲫
胚胎
脂肪酸
普安银鲫
总脂
胚胎
脂肪酸
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ISSN1001-4829

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Summary:为了探明普安银鲫在胚胎发育过程中的能量来源、组成变化和利用模式,采用生物化学方法测定普安银鲫的成熟卵和8个胚胎发育时期的总脂与脂肪酸组成变化。结果表明：①普安银鲫胚胎中成熟卵和胚胎不同发育时期的总脂含量随着胚胎的不断发育而降低,成熟卵与受精卵相比,总脂稍有下降,由其干重的18.25%降至17.94%,但差异不显著（P〉0.05）,成熟卵随受精后胚胎发育的推进,其总脂变化呈逐渐降低趋势,在成熟卵和早期胚胎中的主要脂肪酸为C16：0,C18：0,C18：1n-9和C18：2n-6;与成熟卵相比,普安银鲫受精卵的饱和脂肪酸（SFA）与高度不饱和脂肪酸（HUFA）下降,单不饱和脂肪酸（MUFA）升高。②普安银鲫的成熟卵和胚胎在发育过程中共检测到20种脂肪酸,胚胎对不同脂肪酸的利用率因胚胎发育时期的不同而有差异,SFA在囊胚期与出膜前期升高,其他时期均表现为不同程度的降低;MUFA呈波浪式上升趋势;HUFA呈波浪式降低趋势,但原肠胚期到肌肉效应期HUFA不断升高,到出膜前期有所下降。
Bibliography:51-1213/S
The composition of total lipids and fatty acids in mature eggs and eight stages of embryonic development of C.auratus were analyzed using biochemical methods to explore the energy source,composition change and use model of C.auratus in the embryonic development process.The results showed that：（i） The total lipid content in mature eggs and different stages of C.auratus decreased with the embryonic development.The total lipid content in mature egg decreased from 18.25 %（dry weight） to 17.94 % in comparison of fertilized ovum（P0.05）.The total lipid content decreased gradually with the advancement of embryonic development.The main fatty acids in mature egg and early embryo were C16：0,C18：0,C18：1n-9 and C18：2n-6.Compared with mature egg,the contents of SFA and HUFA decreased while MUFA increased.（ii） A total of 20 fatty acids were detected in mature egg and embryo in the development stages.The use ratio of different fatty acids varied with the variation of development stages.SFA content went up in blastul
ISSN:1001-4829