Efficient Newly Designed Primers for the Amplification and Sequencing of Bird Mitochondrial Genomes

• Published in: Bioscience, biotechnology, and biochemistry Vol. 77; no. 3; pp. 577 - 581
• Main Authors: AMER, Sayed A. M., AHMED, Mohamed Mohamed, SHOBRAK, Mohammed
• Format: Journal Article
• Language: English
• Published: England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013; Oxford University Press
• Subjects: Animals; Aves; birds; Birds - genetics; Conservation of Natural Resources; DNA Primers - genetics; DNA, Mitochondrial - genetics; Genetic Markers - genetics; Genetic Variation; Genome, Mitochondrial - genetics; mitochondrial genome; Nucleic Acid Amplification Techniques - methods; PCR; primer design; Sequence Analysis, DNA - methods
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.120819

In the present study, 27 mitochondrial genomes of diverse avian supra-orders were collected from the Genbank database and their genes were aligned separately. From the alignments, the conserved sequences were selected to design novel conserved primers for amplification and sequencing of the different mitochondrial genes. The reproducibility of these primers was tested in the amplification and sequencing of diverse avian supra-order mitochondrial genomes and was confirmed. This method helped in designing a new set of primers to accelerate both the amplification and the sequencing of bird mitogenomes. It also aids in building mitogenome markers in studying the genetic framework of endemic birds as a preliminary strategy for conservation management of them.