Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan

• Published in: Bioscience, biotechnology, and biochemistry Vol. 77; no. 3; pp. 534 - 543
• Main Authors: PORCIUNCULA, Juliano de Oliveira, FURUKAWA, Takanori, MORI, Kazuki, SHIDA, Yosuke, HIRAKAWA, Hideki, TASHIRO, Kosuke, KUHARA, Satoru, NAKAGAWA, Satoshi, MORIKAWA, Yasushi, OGASAWARA, Wataru
• Format: Journal Article
• Language: English
• Published: England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013; Oxford University Press
• Subjects: Amino Acid Sequence; Ascomycetes; cellulase; Cellulase - biosynthesis; Cellulose - metabolism; comparative genomic analysis; DNA, Fungal - metabolism; filamentous fungi; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes, Fungal - genetics; Glucose - metabolism; Hypocrea jecorina; Japan; Molecular Sequence Data; Mutagenesis; Mutation; next generation sequencing; Polymorphism, Single Nucleotide; Protein Structure, Tertiary; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma - metabolism; Trichoderma reesei; Japan
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.120794

The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of single-nucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.