Catalytic ferrous iron in amniotic fluid as a predictive marker of human maternal-fetal disorders

• Published in: Journal of Clinical Biochemistry and Nutrition Vol. 56; no. 1; pp. 57 - 63
• Main Authors: Hattori, Yuka, Mukaide, Takahiro, Jiang, Li, Kotani, Tomomi, Tsuda, Hiroyuki, Mano, Yukio, Sumigama, Seiji, Hirayama, Tasuku, Nagasawa, Hideko, Kikkawa, Fumitaka, Toyokuni, Shinya
• Format: Journal Article
• Language: English
• Published: Japan SOCIETY FOR FREE RADICAL RESEARCH JAPAN 01.01.2015; Japan Science and Technology Agency; the Society for Free Radical Research Japan
• Subjects: amniotic fluid; catalytic ferrous iron; Original; oxidative stress; pregnancy; catalytic ferrous iron; oxidative stress; pregnancy; amniotic fluid
• ISSN: 0912-0009; 1880-5086
• DOI: 10.3164/jcbn.14-82

Amniotic fluid contains numerous biomolecules derived from fetus and mother, thus providing precious information on pregnancy. Here, we evaluated oxidative stress of human amniotic fluid and measured the concentration of catalytic Fe(II). Amniotic fluid samples were collected with consent from a total of 89 subjects in Nagoya University Hospital, under necessary medical interventions: normal pregnancy at term, normal pregnancy at the 2nd trimester, preterm delivery with maternal disorders but without fetal disorders, congenital diaphragmatic hernia, fetal growth restriction, pregnancy-induced hypertension, gestational diabetes mellitus, Down syndrome and trisomy 18. Catalytic Fe(II) and oxidative stress markers (8-hydroxy-2'-deoxyguanosine, 8-OHdG; dityrosine) were determined with RhoNox-1 and specific antibodies, respectively, using plate assays. Levels of 8-OHdG and dityrosine were higher in the 3rd trimester compared with the 2nd trimester in normal subjects, and the abnormal groups generally showed lower levels than the controls, thus suggesting that they represent fetal metabolic activities. In contrast, catalytic Fe(II) was higher in the 2nd trimester than the 3rd trimester in the normal subjects, and overall the abnormal groups showed higher levels than the controls, suggesting that high catalytic Fe(II) at late gestation reflects fetal pathologic alterations. Notably, products of H2O2 and catalytic Fe(II) remained almost constant in amniotic fluid.