Retinal Function and Morphology in the Rabbit Eye after Intravitreal Injection of the TNF Alpha Inhibitor Adalimumab

• Published in: Current eye research Vol. 39; no. 11; pp. 1106 - 1116
• Main Authors: Myers, Anna Cardiakidis, Ghosh, Fredrik, Andréasson, Sten, Ponjavic, Vesna
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare USA, Inc 01.11.2014; Taylor & Francis
• Subjects: Adalimumab; Animals; Anti-Inflammatory Agents - toxicity; Antibodies, Monoclonal, Humanized - toxicity; Calbindins - metabolism; Clinical Medicine; drug toxicity; electrophysiology; Electroretinography; Fluorescent Antibody Technique, Indirect; Glial Fibrillary Acidic Protein - metabolism; histopathology; intravitreal injection; Intravitreal Injections; Klinisk medicin; Medical and Health Sciences; Medicin och hälsovetenskap; Oftalmologi; Ophthalmology; Original; Parvalbumins - metabolism; Protein Kinase C-alpha - metabolism; rabbit retina; Rabbits; Retina - drug effects; Retina - metabolism; Retina - physiopathology; retinal function; Rhodopsin - metabolism; Tumor Necrosis Factor-alpha - antagonists & inhibitors; tumor necrosis factor-alpha inhibitor; rabbit retina; electrophysiology; drug toxicity; histopathology; intravitreal injection; tumor necrosis factor-alpha inhibitor; Adalimumab; retinal function
• ISSN: 0271-3683; 1460-2202
• DOI: 10.3109/02713683.2014.898309

Abstract Aim: To study the effects of the tumor necrosis factor alpha inhibitor adalimumab on rabbit retina after injection into the vitreous body. Methods: Forty-eight rabbits of mixed strain (9-12 months old, weighing   3.5 kg) were randomized into four groups. Adalimumab was injected at one of two concentrations (1.25 mg or 2.5 mg) into the eyes of two groups, and balanced salt solution into the eyes of the third group. The fourth group acted as controls. Full-field electroretinography (ffERG) was performed before injection and 1 and 6 weeks post-injection. At 6 weeks post-injection the rabbits were euthanized and the sectioned retinas were studied. Retinal histology was studied with hematoxylin-eosin staining. Immunohistochemical analysis was performed on rods, cones, rod bipolar cells, horizontal cells, amacrine cells and Müller cells. Results: No significant difference in ffERG amplitudes or implicit times was observed between the four groups at any time point. Histological and immunohistochemical findings were similar in all groups. Conclusions: Injection of adalimumab into the vitreous body of healthy rabbits, at doses up to 2.5 mg, does not appear to be toxic to the rabbit retina.