GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier
Every organ in the body requires blood vessels for efficient delivery of oxygen and nutrients, but independent vascular beds are highly specialized to meet the individual needs of specific organs. The vasculature of the brain is tightly sealed, with blood-brain barrier (BBB) properties developing co...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 14; pp. 5759 - 5764 |
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Main Authors | , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
05.04.2011
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Every organ in the body requires blood vessels for efficient delivery of oxygen and nutrients, but independent vascular beds are highly specialized to meet the individual needs of specific organs. The vasculature of the brain is tightly sealed, with blood-brain barrier (BBB) properties developing coincident with neural vascularization. G protein-coupled receptor 124 (GPR124) (tumor endothelial marker 5, TEM5), an orphan member of the adhesion family of G protein-coupled receptors, was previously identified on the basis of its overexpression in tumor vasculature. Here, we show that global deletion or endothelial-specific deletion of GPR124 in mice results in embryonic lethality associated with abnormal angiogenesis of the forebrain and spinal cord. Expression of GPR124 was found to be required for invasion and migration of blood vessels into neuroepithelium, establishment of BBB properties, and expansion of the cerebral cortex. Thus, GPR124 is an important regulator of neurovasculature development and a potential drug target for cerebrovascular diseases. |
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Bibliography: | http://dx.doi.org/10.1073/pnas.1017192108 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 1M.C., M.K.E., S.S., and E.Z. contributed equally to this work. Edited* by Bert Vogelstein, Johns Hopkins University, Baltimore, MD, and approved February 23, 2011 (received for review November 16, 2010) Author contributions: M.C., M.K.E., L.T., and B.S.C. designed research; M.C., M.K.E., S.S., E.Z., J.S., M.Y.Y., X.L., A.C., L.X., M.B.H., D.L., E.H., E.V.S., F.C., D.C.H., K.N., and B.S.C. performed research; M.C., M.K.E., S.S., E.Z., D.C.H., K.N., and B.S.C. analyzed data; and M.C. and B.S.C. wrote the paper. |
ISSN: | 0027-8424 1091-6490 1091-6490 |
DOI: | 10.1073/pnas.1017192108 |