Mechanical Wounding of Yeast-Like Conidium Cells of Tremella fuciformis Makes Them Susceptible to Agrobacterium-Mediated Transformation

We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.

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Bibliographic Details
Published inBioscience, biotechnology, and biochemistry Vol. 77; no. 10; pp. 2157 - 2159
Main Authors SHIN, Dong-Il, PARK, Hee-Sung
Format Journal Article
LanguageEnglish
Published England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013
Oxford University Press
Subjects
Agrobacterium
Agrobacterium - genetics
Basidiomycota - cytology
Basidiomycota - genetics
Genetic Engineering - methods
Mechanical Phenomena
mechanical wounding
Spores, Fungal - cytology
transformation
Transformation, Genetic
Tremella fuciformis
yeast-like conidia
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Summary:We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.130473