Assessment of different functionalization methods for grafting a protein to an alumina-covered biosensor

• Published in: Biofabrication Vol. 6; no. 3; p. 035007
• Main Authors: Van Overstraeten-Schlögel, N, Lefèvre, O, Couniot, N, Flandre, D
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.09.2014
• Subjects: alumina; Aluminum oxide; Aluminum Oxide - chemistry; Assessments; Biochemistry - instrumentation; Biochemistry - methods; biofunctionalization; Biomolecules; Biosensing Techniques - instrumentation; Biosensors; Covering; Grafting; Maleimides - chemistry; methods; protein; Proteins; Proteins - chemistry; Respiratory Syncytial Viruses - chemistry; Respiratory Syncytial Viruses - isolation & purification; Selective binding; sensor
• ISSN: 1758-5082; 1758-5090
• DOI: 10.1088/1758-5082/6/3/035007

The specificity of biosensors is typically obtained by surface biofunctionalization, which enables the selective binding of biomolecules. This critical step is sensitive to the nature of materials and to the overall experimental conditions. Here, we provide a comprehensive study of several biofunctionalization methods, including the layer-by-layer technique and both the gas-phase and liquid-phase silanizations, and we propose a new maleimide-based protocol for grafting a protein to a sensor covered by alumina. This method was then validated by making a respiratory syncitial virus-specific biosensor.