Genetic Improvement of Bacillus licheniformis Strains for Efficient Deproteinization of Shrimp Shells and Production of High-Molecular-Mass Chitin and Chitosan

• Published in: Applied and Environmental Microbiology Vol. 76; no. 24; pp. 8211 - 8221
• Main Authors: Hoffmann, Kerstin, Daum, Gabriele, Köster, Marina, Kulicke, Werner-Michael, Meyer-Rammes, Heike, Bisping, Bernward, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2010; American Society for Microbiology (ASM)
• Subjects: Animals; Bacillus - genetics; Bacillus - metabolism; Bacillus licheniformis; Bacteria; Biochemistry; Biological and medical sciences; Biotechnology; Carbohydrates; Chitin; Chitin - chemistry; Chitin - isolation & purification; Chitin - metabolism; chitinase; chitosan; Chitosan - chemistry; Chitosan - isolation & purification; Chitosan - metabolism; Chromatography, Gel; Fermentation; Fundamental and applied biological sciences. Psychology; gel chromatography; Genes, Bacterial; genetic improvement; Genetics; Genotype & phenotype; lipids; Lipids - analysis; Microbiology; Molecular Weight; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; operon; Organisms, Genetically Modified - genetics; Organisms, Genetically Modified - metabolism; Penaeidae - microbiology; Polyglutamic Acid - metabolism; proteolysis; Saccharides; Sequence Deletion; Shellfish; shrimp; Spectrum analysis; viscometry; viscosity; Bacillus licheniformis; Chitin; Bacillaceae; Deproteinization; Macrura; Crustacea; Bacillales; Arthropoda; Genetic improvement; Production; Bacteria; Decapoda; Chitosan; Invertebrata; Molecular mass; Shrimp

By targeted deletion of the polyglutamate operon (pga) in Bacillus licheniformis F11, a derivative form, F11.1 (Δpga), was obtained that, along with lacking polyglutamate (PGA) formation, displayed enhanced proteolytic activities. The phenotypic properties were maintained in a strain in which the ch...