The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher L...

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Published inBioscience, biotechnology, and biochemistry Vol. 77; no. 11; pp. 2309 - 2311
Main Authors YAO, Yuki, SAKAMOTO, Takaiku, HONDA, Yoichi, KAGOTANI, Yasuyuki, IZUMITSU, Kousuke, SUZUKI, Kazumi, IRIE, Toshikazu
Format Journal Article
LanguageEnglish
Published England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013
Oxford University Press
Subjects
Copper Sulfate - pharmacology
Coumaric Acids - pharmacology
Culture Media
Cyclic AMP - biosynthesis
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
GTP-Binding Protein alpha Subunits - genetics
GTP-Binding Protein alpha Subunits - metabolism
laccase
Laccase - biosynthesis
Laccase - genetics
lignin
manganese peroxidase
mushroom
Mutation
Pleurotus - drug effects
Pleurotus - enzymology
Pleurotus - genetics
Pleurotus ostreatus
Transformation, Genetic
Transgenes
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Summary:Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO 4 and ferulic acid.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.130470