Identification of novel autophagy-related lncRNAs associated with a poor prognosis of colon adenocarcinoma through bioinformatics analysis

• Published in: Scientific reports Vol. 11; no. 1; pp. 8069 - 12
• Main Authors: Wu, Dejun, Yin, Zhenhua, Ji, Yisheng, Li, Lin, Li, Yunxin, Meng, Fanqiang, Ren, Xiaohan, Xu, Ming
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.04.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/208; 631/67; Adenocarcinoma; Adenocarcinoma - genetics; Adenocarcinoma - mortality; Adenocarcinoma - pathology; Autophagy; Autophagy - genetics; Bioinformatics; Biomarkers, Tumor - genetics; Colon; Colonic Neoplasms - genetics; Colonic Neoplasms - mortality; Colonic Neoplasms - pathology; Colorectal cancer; Computational Biology - methods; Female; Gene Expression Regulation, Neoplastic; Genomes; Humanities and Social Sciences; Humans; Male; Medical prognosis; multidisciplinary; Phagocytosis; Prognosis; Risk groups; RNA, Long Noncoding - genetics; Science; Science (multidisciplinary); Tumorigenesis; Vascular endothelial growth factor
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-87540-0

LncRNAs play a pivotal role in tumorigenesis and development. However, the potential involvement of lncRNAs in colon adenocarcinoma (COAD) needs to be further explored. All the data used in this study were obtained from The Cancer Genome Atlas database, and all analyses were conducted using R software. Basing on the seven prognosis-related lncRNAs finally selected, we developed a prognosis-predicting model with powerful effectiveness (training cohort, 1 year: AUC = 0.70, 95% Cl = 0.57–0.78; 3 years: AUC = 0.71, 95% Cl = 0.6–0.8; 5 years: AUC = 0.76, 95% Cl = 0.66–0.87; validation cohort, 1 year: AUC = 0.70, 95% Cl = 0.58–0.8; 3 years: AUC = 0.73, 95% Cl = 0.63–0.82; 5 years: AUC = 0.68, 95% Cl = 0.5–0.85). The VEGF and Notch pathway were analyzed through GSEA analysis, and low immune and stromal scores were found in high-risk patients (immune score, cor =  − 0.15, P  < 0.001; stromal score, cor =  − 0.18, P  < 0.001) , which may partially explain the poor prognosis of patients in the high-risk group. We screened lncRNAs that are significantly associated with the survival of patients with COAD and possibly participate in autophagy regulation. This study may provide direction for future research.