A benzothiadiazole primes parsley cells for augmented elicitation of defense responses

• Published in: Plant physiology (Bethesda) Vol. 117; no. 4; pp. 1333 - 1339
• Main Authors: Katz, V.A. (University of Kaiserslautern, Kaiserslautern, Germany.), Thulke, O.U, Conrath, U
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.08.1998
• Subjects: AGENT PATHOGENE; Agronomy. Soil science and plant productions; AMONIO FENILALANINA LIASA; ARN MENSAJERO; ARN MESSAGER; AZOLE; AZOLES; Biological and medical sciences; Cell culture techniques; COUMARINE; COUMARINS; Cultured cells; CUMARINAS; DEFENCE MECHANISMS; DISEASE RESISTANCE; DURACION; DURATION; DUREE; EXPRESION GENICA; EXPRESSION DES GENES; FITOALEXINA; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; GENE; GENE EXPRESSION; GENES; Genetics and breeding of economic plants; INDUCED RESISTANCE; L cells; MECANISME DE DEFENSE; MECANISMOS DE DEFENSA; MESSENGER RNA; ORGANISMOS PATOGENOS; Parsley; PATHOGENS; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; PEROXIDASAS; PEROXIDASES; PEROXYDASE; Pest resistance; PETROSELINUM CRISPUM; PHENYLALANINE AMMONIA LYASE; PHYTOALEXINE; PHYTOALEXINS; Phytopathology. Animal pests. Plant and forest protection; Plant pathogens; Plant-Microbe and Plant-Insect Interactions; Plants; Pretreatment; RESISTANCE AUX MALADIES; RESISTANCE INDUITE; RESISTENCIA A LA ENFERMEDAD; RESISTENCIA INDUCIDA; SECRECION; SECRETION; Varietal selection. Specialized plant breeding, plant breeding aims; Phytophthora sojae; Phenylalanine ammonia-lyase; Mycosis; Plant pathogen; Phycomycetes; Lyases; Activation; Genetic determinism; Fungi; Signal transduction; Disease resistance; Dicotyledones; Angiospermae; Elicitation; Pretreatment; Thallophyta; Salicylic acid; Enzyme; Secretion; Petroselinum crispum; Coumarin; Carbon-nitrogen lyases; Infection; Ammonia-lyases; Sensitivity resistance; Phytoalexin; Flavoring crop; Defense mechanism; Spermatophyta; Umbelliferae; Acquired tolerance
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.117.4.1333

Systemic acquired resistance is an important component of the disease-resistance arsenal of plants, and is associated with an enhanced potency for activating local defense responses upon pathogen attack. Here we demonstrate that pretreatment with benzothiadiazole (BTH), a synthetic activator of acquired resistance in plants, augmented the sensitivity for low-dose elicitation of coumarin phytoalexin secretion by cultured parsley (Petroselinum crispum L.) cells. Enhanced coumarin secretion was associated with potentiated activation of genes encoding Phe ammonia-lyase (PAL). The augmentation of PAL gene induction was proportional to the length of pretreatment with BTH, indicating time-dependent priming of the cells. In contrast to the PAL genes, those for anionic peroxidase were directly induced by BTH in the absence of elicitor, thus confirming a dual role for BTH in the activation of plant defenses. Strikingly, the ability of various chemicals to enhance plant disease resistance correlated with their capability to potentiate parsley PAL gene elicitation, emphasizing an important role for defense response potentiation in acquired plant disease resistance