Abundant Synthesis of Netrin-1 in Satellite Cell-Derived Myoblasts Isolated from EDL Rather Than Soleus Muscle Regulates Fast-Type Myotube Formation

• Published in: International journal of molecular sciences Vol. 22; no. 9; p. 4499
• Main Authors: Suzuki, Takahiro, Mori, Aika, Maeno, Takahiro, Arimatsu, Rio, Ichimura, Emi, Nishi, Yuriko, Hisaeda, Kouga, Yamaya, Yuki, Kobayashi, Ken, Nakamura, Mako, Tatsumi, Ryuichi, Ojima, Koichi, Nishimura, Takanori
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.04.2021; MDPI
• Subjects: Biomedical materials; Cell adhesion & migration; Cell differentiation; Cell membranes; Differentiation; fast-twitch; Mammals; Muscles; Musculoskeletal system; Myoblasts; myofiber type; Myosin; myosin heavy chain; myotube; Myotubes; Netrin-1; Neurogenesis; Osteogenesis; Protein expression; Proteins; Regulation; Satellite cells; Semaphorins; Soleus muscle; Stem cells; myofiber type; myosin heavy chain; myotube; fast-twitch; netrin-1; satellite cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22094499

Resident myogenic stem cells (satellite cells) are attracting attention for their novel roles in myofiber type regulation. In the myogenic differentiation phase, satellite cells from soleus muscle (slow fiber-abundant) synthesize and secrete higher levels of semaphorin 3A (Sema3A, a multifunctional modulator) than those derived from extensor digitorum longus (EDL; fast fiber-abundant), suggesting the role of Sema3A in forming slow-twitch myofibers. However, the regulatory mechanisms underlying fast-twitch myotube commitment remain unclear. Herein, we focused on netrin family members (netrin-1, -3, and -4) that compete with Sema3A in neurogenesis and osteogenesis. We examined whether netrins affect fast-twitch myotube generation by evaluating their expression in primary satellite cell cultures. Initially, netrins are upregulated during myogenic differentiation. Next, we compared the expression levels of netrins and their cell membrane receptors between soleus- and EDL-derived satellite cells; only netrin-1 showed higher expression in EDL-derived satellite cells than in soleus-derived satellite cells. We also performed netrin-1 knockdown experiments and additional experiments with recombinant netrin-1 in differentiated satellite cell-derived myoblasts. Netrin-1 knockdown in myoblasts substantially reduced fast-type myosin heavy chain (MyHC) expression; exogenous netrin-1 upregulated fast-type MyHC in satellite cells. Thus, netrin-1 synthesized in EDL-derived satellite cells may promote myofiber type commitment of fast muscles.