Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study

• Published in: Veterinary microbiology Vol. 133; no. 1; pp. 179 - 183
• Main Authors: Nakano, Hitoshi, Kameo, Yuki, Andoh, Kiyohiko, Ohno, Yoshito, Mochizuki, Masami, Maeda, Ken
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.2009; Elsevier
• Subjects: Animals; Antigens, CD; Antigens, CD - metabolism; Antigens, CD - physiology; Biological and medical sciences; blood serum; canine distemper; Canine distemper virus; Canine morbillivirus; Cats; cell lines; Distemper Virus, Canine; Distemper Virus, Canine - growth & development; Distemper Virus, Canine - immunology; Distemper Virus, Canine - isolation & purification; Dogs; Fundamental and applied biological sciences. Psychology; Genotype; growth & development; immune response; immunology; in vitro studies; isolation & purification; Lymphocyte Activation; lymphocyte proliferation; lymphocytes; metabolism; methods; Microbiology; Miscellaneous; neutralization tests; Neutralization Tests - veterinary; physiology; receptors; Receptors, Cell Surface; Receptors, Cell Surface - metabolism; Receptors, Cell Surface - physiology; serodiagnosis; Signal Transduction; Signaling Lymphocytic Activation Molecule Family Member 1; SLAM; validity; veterinary; Virology; Virus Cultivation; Virus Cultivation - methods; Virus Cultivation - veterinary; Canine distemper virus; SLAM; Fissipedia; Carnivora; Microbiology; Paramyxoviridae; Virus; Paramyxovirinae; Mononegavirales; Vertebrata; Mammalia; Morbillivirus; Veterinary; Dog; Lymphocyte
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2008.06.016

Signaling lymphocyte activation molecule (SLAM) is one of the receptors for canine distemper virus (CDV). In this study, canine and feline cells expressing canine SLAM, designated A-72/cSLAM and CRFK/cSLAM, were established for the in vitro study of canine distemper. Recent CDV isolates, KDK-1 and 246, which belong to genotypes Asia/H1 and Asia/H2, respectively, rapidly grew and produced distinct syncytia in both the SLAM-expressing cells. The virus-neutralizing (VN) test was successfully performed using these cells, and the results indicated that sera from dogs experimentally infected with KDK-1 had higher VN titers for homologous strain KDK-1 than for heterologous strain 246 and the vaccine Onderstepoort. These newly established cells expressing canine SLAM would help virological and serological analyses of canine distemper.