Alteration of photosystem II properties with non-photochemical excitation quenching

• Published in: Philosophical transactions of the Royal Society of London. Series B. Biological sciences Vol. 355; no. 1402; pp. 1405 - 1418
• Main Authors: Laisk, A., Oja, V.
• Format: Journal Article
• Language: English
• Published: England The Royal Society 29.10.2000
• Subjects: Antennas; Chlorophylls; Electrons; Energy Transfer; Fluorescence; Light Harvesting and Dissipation Reactions Associated with Electron Transport; Light-Harvesting Protein Complexes; Oxygen; Photochemistry; Photoinhibition; Photoregulation; Photosynthesis; Photosynthesis - physiology; Photosynthetic Reaction Center Complex Proteins - metabolism; Photosystem II; Photosystem II Protein Complex; Plants; Quenching; Sensors
• ISSN: 0962-8436; 1471-2970
• DOI: 10.1098/rstb.2000.0702

E. The more slowly induced but still reversible
type (photoprotective) quenching does not induce changes in the number of active PS II or in the PS II maximum turnover rate, thus confirming the antenna mechanism of
but decreased considerably when exposure to saturating light was extended or O
analyser, combined with a flexible gas system, was used for these measurements. Oxygen yield from saturating single turnover flashes was the equivalent of 1.3–2.0 μmol e
Oxygen yield from single turnover flashes and multiple turnover pulses was measured in sunflower leaves differently pre–illuminated to induce either ‘energy–dependent type’ non–photochemical excitation quenching (
I.
quenching was induced by a 1 min exposure to saturating light, but it decreased when pre–illumination was extended to 30–60 min. Oxygen evolution from saturating multiple turnover pulses behaved similarly: it did not decrease with the rapidly induced
) or reversible, inhibitory type non–photochemical quenching (
type quenching (photoinactivation) induced a decrease in the number of active PS II and in the maximum PS II turnover rate. Modelling showed that, mainly, the acceptor side resistance of PS II increased in parallel with the reversible
evolution during multiple turnover pulses, interpreted with the help of a mathematical model of photosystem II (PS II) electron transport, revealed PS II donor and acceptor side resistances. These experiments showed that PS II properties depend on the type of non–photochemical quenching present. The rapidly induced and rapidly reversible
concentration was decreased to 0.4%. Parallel recording of chlorophyll fluorescence and O
in leaves pre–adapted to low light. It did not decrease when
). A zirconium O