HPLC法测定癸氧喹酯壳聚糖-海藻酸钠微囊中药物含量

建立癸氧喹酯壳聚糖-海藻酸钠微囊中癸氧喹酯的HPLC含量测定方法。采用破囊液（O．2mol／LNaHC03和0．06mol／LNa3c6H507·2H20，pH=7．8—8．2）对癸氧喹酯壳聚糖-海藻酸钠微囊进行破囊，破囊后用甲醇和三氨甲烷混合溶液（4：1）超声提取癸氧喹酯。HPLC检测方法采用AgilentZORBAX80AExtend-C18色谱柱；以乙腈-0．1％甲酸水溶液（75：25）为流动相；检测波长为267nm；流速为1mL／min。结果表明，所采取的破囊方法破解效率商，微囊破解完全。方法精密度与准确度表明，癸氧喹酯浓度在0．05-10μg/mL的范围内线性关系良好，回归方程为Y=...

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Published in西南农业学报 Vol. 26; no. 4; pp. 1486 - 1489
Main Author 鄂伟 张启迪 王君玮 曲志娜 赵思俊 邹明
Format Journal Article
LanguageChinese
Published 青岛农业大学动物科技学院,山东青岛,266109%中国动物卫生与流行病学中心,山东青岛,266032 2013
Subjects
亮聚糖-海藻酸钠微囊
商效液相色谱法
癸氧喹酯
壳聚糖-海藻酸钠微囊
高效液相色谱法
Decoquinate
Chitosan-alginate microcapsules
癸氧喹酯
HPLC
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ISSN1001-4829

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Summary:建立癸氧喹酯壳聚糖-海藻酸钠微囊中癸氧喹酯的HPLC含量测定方法。采用破囊液（O．2mol／LNaHC03和0．06mol／LNa3c6H507·2H20，pH=7．8—8．2）对癸氧喹酯壳聚糖-海藻酸钠微囊进行破囊，破囊后用甲醇和三氨甲烷混合溶液（4：1）超声提取癸氧喹酯。HPLC检测方法采用AgilentZORBAX80AExtend-C18色谱柱；以乙腈-0．1％甲酸水溶液（75：25）为流动相；检测波长为267nm；流速为1mL／min。结果表明，所采取的破囊方法破解效率商，微囊破解完全。方法精密度与准确度表明，癸氧喹酯浓度在0．05-10μg/mL的范围内线性关系良好，回归方程为Y=61．768X＋2．8814，R0=0．9996。本方法具有操作简便、快速、准确可靠等特点，适合癸氧喹确壳聚糖-海藻酸钠微囊中药物含量测定。
Bibliography:E WeiI , ZHANG Qi-diI ,WANG Jun-wei2, QU Zhi-na2 , ZHAO Si-jun2, ZOU MingI ＊ （ 1. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Shandeng Qingdan 266109 ,China; 2. China Ani- ms] Health and Epidemiology Center, Shandong Qingdao 266032, China）
HPLC ; Deeoquinate; Chitosan-alginate microcapsules
51-1213/S
A high pedormance liquid chromatographic method was developed for determining decoquinate in chitoasn-alginate microcapsules. The chitosan-alginate mlcrocapsules were broken by the microcapsu]e-breken solution （0. 2 moL/L NaHCO3 and 0.06 mol/L Na3 Ce H5 07· 2 Hz O, pH = 7.8 - 8.2）, and the decoquinate was extracted using methanol-trich]oromethane mixed solution （ 4 ： 1 ） by ultrasonic. HPLC detection method was adopted with Agilent ZORBAX g0A Extend-Cls colurrm, the mobile phase of acetonitrile -0.1% formic acid （75 ： 25 ）, the detection wavelength of 267 nm and the flow rate of 1 nd.,/min. The result showed that because of HLPC method with precision and accuracy, cibration pl
ISSN:1001-4829