Phagocytosis Conditions of Peripheral Blood Leucocytes in Chicken

• Published in: Animal husbandry and feed science Vol. 2; no. 1; pp. 14 - 16
• Main Authors: Qin, F, Yu, S-L, Chen, Y-Y, Fang, X-X, Yu, J
• Format: Journal Article
• Language: English
• Published: 2010
• Subjects: Staphylococcus; 吞噬作用; 吞噬指数; 吞噬活性; 外周血白细胞; 最佳条件; 正交试验法; 葡萄球菌属; 鸡
• ISSN: 1943-9911

[Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphy/ococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken＇s peripheral blood leucocytes was detected by the method of Wright＇s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages （PP） and phagocytosis index （PI） were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [ Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41℃, time of 40 min and concentration of Staphylococcus spp. 10 145 of 5 ×10^ 6/ml. The order of various factors on leucocytes phagocytcsis index was the concentration of Staphylococcus spp. 10 145 〉 time 〉 temperature. [ Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.