Effect of Various Factors on Orange II Decoloration Reaction by Horseradish Peroxidase in the Presence of Detergent Enzymes

• Published in: Journal of Oleo Science Vol. 67; no. 3; pp. 315 - 319
• Main Authors: Morita, Miyuki, Tani, Michiko, Onikoshi, Mayuko, Mase, Kiyomi, Masuko, Fumi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Japan Oil Chemists' Society 2018; Japan Science and Technology Agency
• Subjects: Azo Compounds - chemistry; Benzenesulfonates - chemistry; Bleaching Agents; Ca and Mg ion; Calcium - chemistry; Calcium ions; Color; decoloration reaction; Decoloring; detergent enzymes; Detergent industry; Detergents - chemistry; Enzymes; Enzymes - chemistry; horseradish peroxidase; Horseradish Peroxidase - chemistry; Hydrogen-Ion Concentration; Ions; Magnesium - chemistry; Orange II; Peroxidase; Serine Endopeptidases - chemistry; Ca and Mg ion; decoloration reaction; Orange II; detergent enzymes; horseradish peroxidase
• ISSN: 1345-8957; 1347-3352
• DOI: 10.5650/jos.ess17103

The effects of four detergent enzymes on the Orange II decoloration reaction by horseradish peroxidase (HRP) were investigated. Stainzyme, Lipex, Celluclean, and Savinase did not affect the decoloration reaction up to a concentration of 0.1% Under weak alkaline to alkaline conditions, the presence of four enzymes did not affect the decoloration reaction, but affected the decoloration reaction under neutral to acidic conditions. The effect of Ca2+ and Mg2+ concentrations on Orange II decoloration reactions in the presence of four enzymes was also investigated. Ca2+ and Mg2+ did not affect the decoloration reaction up to 500 ppm.