Effects of high cholesterol diet on newly generated cells in the dentate gyrus of C57BL/6N and C3H/HeN mice

• Published in: Journal of Veterinary Medical Science Vol. 71; no. 6; pp. 753 - 758
• Main Authors: Kim, I.Y.(Seoul National Univ. (Korea R.)), Hwang, I.K, Choi, J.W, Yoo, K.Y, Kim, Y.N, Yi, S.S, Won, M.H, Lee, I.S, Yoon, Y.S, Seong, J.K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.06.2009; Japan Science and Technology Agency
• Subjects: Animals; BRAIN; C3H/HeN; C57BL/6N; CELL DIFFERENTIATION; Cell Proliferation - drug effects; CELLS; CELLULE; CELULAS; CEREBRO; CHOLESTEROL; Cholesterol, Dietary - administration & dosage; Cholesterol, Dietary - metabolism; COLESTEROL; Dentate Gyrus - cytology; Dentate Gyrus - drug effects; Dentate Gyrus - metabolism; DIET; DIETA; DIFERENCIACION CELULAR; DIFFERENCIATION CELLULAIRE; doublecortin; ENCEPHALE; Female; high cholesterol diet; Hypercholesterolemia - metabolism; Hypercholesterolemia - pathology; Immunohistochemistry; IMMUNOLOGICAL TECHNIQUES; Ki-67 Antigen - metabolism; Ki67; Male; MICE; Mice, Inbred C3H; Mice, Inbred C57BL; Microtubule-Associated Proteins - metabolism; Neurogenesis - physiology; Neuropeptides - metabolism; RATON; REGIME ALIMENTAIRE; SOURIS; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.71.753

In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.